Ft nmr avance 3 hd

All reagents and solvents were obtained from commercial suppliers and used as received unless otherwise specified. All reactions were performed under nitrogen atmosphere using oven-dried glassware and monitored by thin-layer chromatography (TLC) on a silica-coated plate (60 F254, Merck, Darmstadt, Germany). Separated compounds on the TLC plate were visualized under UV light at 254 nm and 365 nm (VL-4.LC, Vilber Lourmat, Eberhardzell, Germany). Column chromatography was carried out using a silica gel 230-400 mesh (ZEOprep, Zeochem, Lake Zurich, Switzerland) with n-hexane, EtOAc, CH₂Cl₂ and MeOH as eluents. ¹H NMR (400 MHz) and ¹³C NMR (125 MHz) spectra were recorded using a FT-NMR Avance III HD (Bruker, Billerica, MA, USA) at ambient temperature. Chemical shifts were reported in ppm (parts per million) relative to tetramethylsilane and coupling constants (J) were expressed in hertz (Hz). The following abbreviations are used for multiplicities: s = singlet; brs = broad singlet; d = doublet; t = triplet; q = quartet; m = multiplet; dd = doublet of doublets. Mass-to-charge ratio (m/z) values were obtained by using high-resolution mass spectrometry (HRMS) under fast atom bombardment (FAB) conditions with a JMS-700 MStation (JEOL, Tokyo, Japan).

All the chemicals and solvents were purchased from Merck (Merck KGaA, Darmstadt, Germany) in the highest purity grade available and used without further purification unless otherwise specified. Nuclear Magnetic Resonance (NMR) spectra were recorded on a Bruker Biospin FT-NMR AVANCE III HD (600 MHz) spectrometer equipped with a CryoProbe BBO H&F 5 mm in inverse detection (Bruker corporation 40 Manning Road Billerica, MA, USA). The nominal frequencies were 150.90 MHz for ¹³C and 600.13 MHz for ¹H, respectively.