Nisin staining solution

Para-chlorophenylalanine (PCPA) was purchased from Shanghai Genye. The 0.9% NaCl solution was adjusted to a weakly basic pH (pH 7.0–8.0) with NaOH and HCl to prepare a PCPA suspension (5% Tween-80) for use in this study. 7,8-Dihydroxyflavone (7,8-DHF) was purchased from MCE, pentobarbital sodium was purchased from Sigma Merck, dimethyl sulfoxide (DMSO) was purchased from Solepipe, and PEG300 was purchased from MCE. Eosin was purchased from Solarbio, hematoxylin was purchased from Servicebio, nisin staining solution was purchased from Servicebio, neutral resin was purchased from Shanghai Sinopharm, the In Situ Cell Death Detection Kit, the DAB concentrated kit was purchased from Solarbio, hematoxylin was purchased from Servicebio, the Marker was purchased from Helix, and the BCA protein concentration assay kit was purchased from Solarbio. ELISA kits for interleukin-1β (IL-1β), prostaglandins D2 (PGD2), melatonin (MT), corticosterone (CORT), IL-6, IL-10, tumor necrosis factor alpha (TNF-α), interferon-gamma (IFN-γ), and brain-derived neurotrophic factor (BDNF) were purchased from Bioswamp. TRIzol was purchased from Ambion, and PrimeScript II Rtase was purchased from TAKARA. Antibodies against Bcl-2, Bax, Bad, Caspase-3, TrKB, PI3K, Akt, cAMP, CREB, BDNF, GAPDH, p-TrKB, p-Akt and p-CREB, as well as goat anti-rabbit IgG, were purchased from CST.