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3 protocols using mouse anti gapdh am4300

1

Antibody Verification for Protein Studies

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Goat anti-FE65 (E-20), mouse anti-ARF6 (3A-1), mouse anti-ARNO (H-7), mouse anti-c-Jun (D-11) and mouse anti-α-Tubulin (DM1A) were purchased from Santa Cruz. Rabbit anti-ARNO, rabbit anti-GFP, rabbit anti-His and rabbit anti-FLAG were obtained from Proteintech. Mouse anti-myc antibody (9B11) and rabbit anti-COX IV (3E11) were obtained from Cell Signaling Technology. Mouse anti-pan-cadherin (C1821), mouse anti-β-COP (maD) and mouse anti-FLAG antibody (M2) were obtained from Sigma. Goat anti-GST antibody was obtained from GeneTex. Mouse anti-GAPDH (AM4300) was purchased from Ambion. Rabbit anti-β-Tubulin was purchased from Abcam. Rabbit anti-FE65 was as previously described [13 (link),14 (link)]. Rat polyclonal antibodies against ARF6, ARNO and GST were created by immunization of rats with ARF6, ARNO and GST bacterial proteins, respectively.
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2

Antibodies for THOC5, THOC7, and Markers

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Affinity-purified rabbit polyclonal antibodies against THOC5 and THOC7 were previously described (Katahira et al., 2009) (link). The following antibodies were commercially acquired; mouse anti-GAPDH (AM4300: Ambion), rabbit anti-γH2AX (EP854(2)Y: Epitomics), rabbit anti-mouse IgG (610-4112: Rockland), Alexa-conjugated goat anti-rabbit IgG (Thermo Fisher Scientific), horseradish peroxidase-and alkaline phosphatase-conjugated goat anti-rabbit and antimouse IgG (Bio-Rad).
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3

Antibody Validation for Protein Analysis

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Mouse anti-FLAG M2 was purchased from Sigma (Sigma). Mouse anti-TRIM29 (A-5: sc-166718), rabbit anti-p63 (H-137: sc-8343), mouse anti-p53 (DO-1: sc-126) rabbit anti-Pol II (N-20: sc-899) and mouse anti-actin (C-2: sc-8432) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA.). Rabbit anti-Integrin β4 (4707) was purchased from Cell Signaling Technology (Beverly, MA). Rabbit anti-Integrin β1 (ab52971) was purchased from Abcam (Cambridge, UK). Mouse anti-GAPDH (AM4300) was purchased from Ambion (Austin, TX). Rabbit anti-MED12 was purchased from Bethyl Laboratories (Bethyl Laboratories, Montbomery, TX).
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