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Promix cell labeling mix 35s met plus 35s cys

Manufactured by PerkinElmer
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Promix cell labeling mix ([35S]Met plus [35S]Cys) is a product by PerkinElmer that contains a combination of radioactive methionine and cysteine for metabolic labeling of cellular proteins.

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Lab products found in correlation

Mg132, by Merck Group (2 mentions) Polybrene, by Merck Group (1 mentions) Nocodazole, by Merck Group (1 mentions) Polyethylenimine (pei), by Polysciences (1 mentions) Rainbow 14c labeled methylated protein standards, by GE Healthcare (1 mentions) Protein a sepharose, by Repligen (1 mentions) Tunicamycin tun, by Merck Group (1 mentions)

Spelling variants of this product

35S-Met/Cys (30 citations) 35S-Met (26 citations) [35S]Cys (5 citations)

2 protocols using promix cell labeling mix 35s met plus 35s cys

1

Radiolabeled Protein Standards for Cell Assays

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Rainbow 14C-labeled methylated protein standards were obtained from GE Healthcare (Little Chalfont, United Kingdom). Promix cell labeling mix ([35S]Met plus [35S]Cys), >1000 Ci/mmol, was from PerkinElmer (Boston, MA), protein A–Sepharose from Repligen (Needham, MA), and Lac from Calbiochem (La Jolla, CA). S-protein agarose was from Novagen (Gibbstown, NJ). Polyethylenimine (PEI) was from Polysciences (Eppelheim, Germany). Anti-mouse immunoglobulin G (IgG)–agarose, MG-132, Tun, Puro, nocodazole, Dox, Sal, Polybrene, and other common reagents were from Sigma-Aldrich (St. Louis, MO).
Leitman J., Shenkman M., Gofman Y., Shtern N.O., Ben-Tal N., Hendershot L.M, & Lederkremer G.Z. (2014). Herp coordinates compartmentalization and recruitment of HRD1 and misfolded proteins for ERAD. Molecular Biology of the Cell, 25(7), 1050-1060.
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2

Promix cell labeling and treatment

Cited 1 time
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Promix cell labeling mix ([35S]Met plus [35S]Cys), >1000 Ci/mmol was from PerkinElmer (Boston, MA). MG-132, tunicamycin (Tun), PKR inhibitor (PKRi), poly-I:C, DMEM Cys/Met-free, Trichloroacetic acid (TCA) and other common reagents were from Sigma. Guanabenz (Gz) was a kind gift of Anne Bertolotti. PERK inhibitor A4 [22] (link) was from ChemBridge Chemical Store.
Leitman J., Barak B., Benyair R., Shenkman M., Ashery U., Hartl F.U, & Lederkremer G.Z. (2014). ER Stress-Induced eIF2-alpha Phosphorylation Underlies Sensitivity of Striatal Neurons to Pathogenic Huntingtin. PLoS ONE, 9(3), e90803.
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