Ifkine green donkey anti mouse igg

For immunofluorescence of the spinal cord tissues at 3 dpi, tissue slides and round coverslips were blocked with 5% bovine serum albumin for 1 hour, and then incubated with primary antibody overnight at 4°C. The primary antibodies included mouse anti-platelet-derived growth factor receptor (Pdgfr; 1:250; Cat# ab69506; Abcam), mouse anti-caspase 1 (1:250, Cat# sc-398715, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-Nod1 (1:200; Cat# PA5-79747; Invitrogen, Carlsbad, CA, USA), and rabbit anti-CD31 (1:200; Cat# ab24590; Abcam). After washing with PBS three times, the tissues were incubated with the corresponding secondary antibody (IFKine™ Green donkey anti-mouse IgG, 1:300, Cat# A24211, Abbkine, Wuhan, China; Dylight 594, rabbit anti-goat IgG, 1:300, Cat# A23430, Abbkine) for 1 hour at room temperature in the dark. Subsequently, the tissues were stained with 4′,6-diamidino-2-phenylindole (DAPI) for 15 minutes, and images were acquired using fluorescence microscopy (Olympus).