Sodium chloride (nacl)

Manufactured by Biopack

Sourced in Argentina

Sodium chloride is a chemical compound composed of sodium and chloride ions. It is a white, crystalline solid that is commonly known as salt. Sodium chloride is an essential compound used in various industries, including food processing, water treatment, and pharmaceutical manufacturing.

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Lab products found in correlation

Sodium hydrogencitrate sesquihydrate, by Merck Group (3 mentions) Sodium citrate dihydrate, by Merck Group (3 mentions) Acetonitrile, by Merck Group (3 mentions) K2hpo4, by Biopack (2 mentions) Azoxystrobin, by Merck Group (2 mentions) Epoxiconazole, by Merck Group (2 mentions) Deltamethrin, by Merck Group (2 mentions) Penconazole, by Merck Group (2 mentions) Cyproxonazole, by Merck Group (2 mentions) Anhydrous sodium sulfate, by Biopack (2 mentions) Psa bulk powder, by Agilent Technologies (2 mentions) Kresoxim methyl, by Merck Group (2 mentions) D spe, by Agilent Technologies (2 mentions) Free base l arginine, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit igg, by Promega (1 mentions) Ethanol and methanol, by Merck Group (1 mentions) Dimethylsulfoxide, by Biopack (1 mentions) N butanol, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Resazurin sodium salt, by Merck Group (1 mentions)

10 protocols using sodium chloride (nacl)

Enzymatic Biomass Pretreatment Protocol

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All the enzymes were supplied
by Novozymes A/S, Denmark: an amylase, two serine endoproteases (SE
I and SE II), a cellulase/xylanase, and a peroxidase. Stock solutions
were made in PBS 1× (buffer phosphate solution, pH 7.3–7.5)
from Biopack reagents.
For LB medium preparation, yeast extract
and tryptone were obtained from Britania Laboratories, and sodium
chloride was obtained from Biopack. For M63 minimal medium with 0.4%
arginine, H₂PO_4, K₂HPO₄, and MgSO₄ were provided by Biopack, (NH₄)₂SO₄ was supplied by Anedra, and l-arginine
free base was provided by Sigma-Aldrich. Deionized water was obtained
by a Double Pass Reverse Osmosis System (Wasserteck, Argentina), with
a resistivity of 1.5 MΩ cm. CV and sugar standards for HPAEC
were provided by Sigma-Aldrich.

Levy I.K., Salustro D., Battaglini F., Lizarraga L., Murgida D.H., Agusti R., D’Accorso N., Raventos Segura D., González Palmén L, & Negri R.M. (2024). Quantification of Enzymatic Biofilm Removal Using the Sauerbrey Equation: Application to the Case of Pseudomonas protegens. ACS Omega, 9(9), 10445-10458.

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Characterization of PTEN DNA Aptamers

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PTENz14-106, PTENz7-biotin, PTENz7-106, PTENz7-70, and PS2.M-H1 DNA aptamers were purchased from IDT (Coralville, IA, USA) and Macrogen (Seoul, Korea). Hemin and 3,3’-diaminobenzidine (DAB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). IgG monoclonal PTEN antibodies (sc-20 PTEN Santa Cruz, CA, USA) and HRP-conjugated goat anti-rabbit IgG were purchased from Promega (Madison, WI, USA). Tris(hydroxymethyl) aminomethane (Tris buffer), potassium chloride, sodium chloride, ethylene-diamine-tetraacetic acid (EDTA), dimethyl sulfoxide (DMSO) and hydrogen peroxide 30% (H₂O₂), Tween 20%, absolute ethanol, and xylene were purchased from Biopack (Buenos Aires, Argentina), and Triton X-l00 from Anedra (Buenos Aires, Argentina). Synthetic balsam was purchased from Alwik (Buenos Aires, Argentina). All chemicals were reagent grade and used without further purification. All solutions were prepared with ultra-pure water (Milli-Q).

Longinotti G., Ybarra G., Vighi S., Perandones C., Montserrat J., Yakisich J.S., Grasselli M, & Radrizzani M. (2021). One Step Histological Detection and Staining of the PTEN Tumor Suppressor Protein by a Single Strand DNA. Diagnostics, 11(2), 171.

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Pesticides Analysis in Samples

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The pesticides standards of deltamethrin, penconazole, kresoxim-methyl, cyproxonazole, epoxiconazole and azoxystrobin were supplied by Sigma-Aldrich (Seelze, Germany). The stock solutions (1000 mg/L) were prepared by dissolving the standards in methanol HPLC grade (99.9%) purchased by Sintorgan (Buenos Aires, Argentina), and stored under freezing condition (-18°C ± 1°C) in dark bottles sealed with PTFE/silicone caps. The working standard solutions (50 mg/L) were prepared in acetonitrile (ACN) of high purity grade for residue analysis, provided by Merck (Darmstadt, Germany).
Deionized water obtained from an E-pure water purification system (Barnstead/Thermolyne, Bedford, MA, United States) was used for sample swelling in the extraction step.
For extraction and partitioning, sodium chloride and anhydrous sodium sulfate were purchased from Biopack (Buenos Aires, Argentina); sodium hydrogencitrate sesquihydrate and sodium citrate dihydrate were obtained from Sigma-Aldrich (Seelze, Germany). For clean-up using d-SPE, C18 and PSA bulk powder were obtained from Agilent Technologies (Santa Clara, United States).

Medina M.B., Munitz M.S, & Resnik S.L. (2021). Fate and health risks assessment of some pesticides residues during industrial rice processing in Argentina. Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems, 98.

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Preparation of Growth Media Reagents

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Mueller-Hinton broth, agar, brain-heart infusion, yeast extract, glucose and peptone were obtained from Britania S.A. (Ciudad Autónoma de Buenos Aires, Argentina). Sodium chloride (99.99%) from Alun (San Martin, Buenos Aires, Argentina) and K 2 HPO 4 (98%) from Cicarelli (San Lorenzo, Santa Fe, Argentina) were analytical grade; and sodium pyruvate (≥99%) from Biopack (Zarate, Buenos Aires, Argentina) was extra pure. Distilled water was sterilized in autoclave FAC (Buenos Aires, Argentina). Sterilized polystyrene 96-well culture plates were supplied by Deltalab (Barcelona, Spain). Acetonitrile, ethanol and methanol (HPLC grade), acetic acid (ACS reagent), n-butanol (FCC reagent), and resazurin sodium salt (for cell culture) were supplied by Sigma-Aldrich (Saint Louis, MO, USA); and dimethylsulfoxide (analytical grade), by Biopack (Zarate, Buenos Aires, Argentina).

Giménez-Martínez P., Ramírez-Ambrosi M., Rosa M.A., Gallo B., Luis A.B., Maggi M., & Fuselli S. (2020). Antimicrobial Activity of Phenolic Extract of Apple Pomace againstPaenibacillus larvaeand its Toxicity onApis mellifera. Journal of Apicultural Science, 64(2), 199-208.

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Pesticides Analysis in Samples

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Medina M.B., Munitz M.S., & Resnik S.L. (2021). Fate and health risks assessment of some pesticides residues during industrial rice processing in Argentina. Journal of Food Composition and Analysis, 98, 103823-103823.

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Chitosan-Starch Polymer Synthesis

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Chitosan (CS) of medium molecular weight (81% degree of deacetylation), native potato starch (ST), ethyl alcohol (≥99.5% purity), acetone (≥99.9% purity), glutaraldehyde solution (25% in water) and sodium tripolyphosphate (STPP) (85% purity) were purchased from Sigma-Aldrich (USA). Lactic acid (85% wt/wt) and sodium hydroxide (NaOH) were purchased from Cicarelli (Argentina). Potassium hydroxide (> 85% purity), sodium chloride (99.5% purity) and DL-malic acid (≥99.5% purity) were purchased from Biopack (Argentina). Beef peptone and yeast extract were purchased from Britania (Argentina). Dipotassium hydrogen phosphate was purchased from Merck (Germany). All reagents were of analytical grade and used as received.

Perez J.J., Francois N.J., Maroniche G.A., Borrajo M.P., Pereyra M.A, & Creus C.M. (2018). A novel, green, low-cost chitosan-starch hydrogel as potential delivery system for plant growth-promoting bacteria. Carbohydrate polymers, 202.

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Spray-Drying Encapsulation Protocol

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The encapsulating agent used for spray-drying was maltodextrin DE10 (dextrose equivalent 10-MD10) (Ingredion S.A, Argentina). The salts (analytical grade) used to control the relative humidity (RH %) were magnesium chloride (33%), potassium carbonate (43%), sodium bromide (58%), sodium chloride (75%), and ammonium sulfate (80%), purchased from Biopack (Argentina). The Folin-Ciocalteu reagent was obtained from Merck (Darmstadt, Germany). Standards of gallic acid [149-91-7] and malvidin-3-glucoside chloride [7228-78-6] were supplied by Sigma Aldrich (St. Louis, USA). Formic acid (98%), chromatography grade methanol and acetonitrile were purchased from Merck (Darmstadt, Germany). All reactive chemicals were analytical grade or superior. Ultra-pure water was obtained from a RiO/Elix3-Sinergy185 purification system (Millipore, Sao Pablo, Brazil). Cellulose filters (3 μm pore size) and (0.45 μm pore size) nylon membrane were supplied by Microclar (Buenos Aires, Argentina).

Alvarez Gaona I.J., Fanzone M., Sari S., Assof M., Pérez D., Chirife J, & Zamora M.C. (2019). Spray-dried Ancellotta red wine: natural colorant with potential for food applications. European food research and technology = Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A, 245(12).

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Gustatory Heterogeneity Induction Protocol

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To generate a gustatory heterogeneity in the arena we added (homogeneously with a micropipette) 100 μl of 1 M NaCl over the filter paper covering the floor of the stimulus zone and 100 μl of distilled water on the control zone (inset Figure 5). The NaCl (purchased in Biopack, Argentina) solution was prepared in distilled water. This concentration was chosen as in previous works it was efficient in deterring feeding in the same species (unpublished data). The experiment started immediately after loading the water and the NaCl solution (i.e., 1 min approximately) in order to minimize water evaporation.

Minoli S., Cano A., Pontes G., Magallanes A., Roldán N, & Barrozo R.B. (2018). Learning Spatial Aversion Is Sensory-Specific in the Hematophagous Insect Rhodnius prolixus. Frontiers in Psychology, 9, 989.

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Antifungal Activity Evaluation Protocol

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HPLC and ACS solvents were from Sintorgan Labs (Buenos Aires, Argentina); penicillin, streptomycin, Sabouraud dextrose agar (SDA) and Mueller-Hinton Agar (MH) media from Britania Labs (Buenos Aires, Argentina); Fluconazole, histopaque 1077, 2H-tetrazolium-2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl] hydroxide salt (MTT), menadione, glucose, glutamine, methylene Blue and p-anisaldehyde, Na₂HPO₄ and NaH₂PO₄ from Sigma-Life Science (Saint Louis, MO, USA); KCl, Na₃PO₄ and NaCl from Biopack (Buenos Aires, Argentina); DMSO, glycerol, Silica Gel 60 (less than 0.063 mm) and Trypan Blue from Merck (Darmstadt, Hesse, Germany); sephadex LH20 from Amersham Biosciences, Uppsala, Sweden; RPMI 1640 from Microvet Labs (Buenos Aires, Argentina); CHROMagar® Candida from CHROMagar Microbiology (Paris, France) and silica gel 60 F254 plates from Macherey-Nagel GmbH & Co. KG (Düren, Germany).

Soberón J.R., Sgariglia M.A., Carabajal Torrez J.A., Aguilar F.A., Pero E.J., Sampietro D.A., Fernández de Luco J, & Labadie G.R. (2020). Antifungal activity and toxicity studies of flavanones isolated from Tessaria dodoneifolia aerial parts. Heliyon, 6(10), e05174.

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Pesticide Residues Analysis Protocol

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All pesticides standards were purchased from Sigma-Aldrich (Seelze, Germany). The working standard solutions for pesticide residues analysis (50 mg/L) were prepared in acetonitrile (ACN) of high purity grade, provided by Merck (Darmstadt, Germany), and stored under freezing condition (-18°C ± 1°C) in dark bottles sealed with PTFE/silicone caps.
Anhydrous Na 2 SO 4 and NaCl were obtained from Biopack (Buenos Aires, Argentina); sodium hydrogencitrate sesquihydrate and sodium citrate dihydrate were purchased from Sigma-Aldrich (Seelze, Germany). Primary Secondary Amine (PSA) and C18 were obtained from Agilent Technologies (Santa Clara, United States).

Medina M.B., Resnik S.L, & Munitz M.S. (2021). Optimization of a rice cooking method using response surface methodology with desirability function approach to minimize pesticide concentration. Food chemistry, 352.

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