Fourier transform-infrared (FT-IR) spectra of the drug, each polymer, 1:1 drug–polymer PM and the corresponding KM were recorded using Fourier transform-infrared spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Each sample (2 mg) was ground with potassium bromide (200 mg) and compressed into discs by a hydraulic press to be finally scanned over a wavenumber ranged from 500 to 4000 cm−1.
Fourier transform infrared spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Fourier transform-infrared spectrophotometer is a laboratory instrument that uses infrared light to analyze the chemical composition and molecular structure of materials. It measures the absorption of infrared radiation by a sample, which can provide information about the chemical bonds and functional groups present in the material.
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3 protocols using fourier transform infrared spectrophotometer
1
FT-IR Analysis of Drug-Polymer Blends
2
Characterization of Nanostructured Materials
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XRD (X-ray diffractometer; Panalytical, X’ Pert Pro, using Cu Kα1 radiation) analysis was performed to evaluate the phase characterization of the nanostructures. FTIR (Fourier Transform Infrared Spectrophotometer; Thermo Avatar) was used through KBr pellets in the region between 400 and 4000 cm-1 to study the chemical structure of the powders, while for the filter papers, ATR (the variable angle attenuated total reflectance) mode was taken in the region between 600 and 4000 cm-1. The morphology of the particles and filters was imaged using a field-emission scanning electron microscopy equipped with an EDS energy dispersive spectroscopy (FE-SEM; TESCAN MIRA 3) and a transmission electron microscope (TEM; Zeiss-EM10C-100 kV). The zeta potential of the filters was studied through an electrokinetic analyzer (SurPASS, Anton Paar Ltd, Austria) using a 1 mM KCl electrolyte solution. To evaluate the surface charge of the particles (dispersed in distilled water), a Horiba SZ-100 Zeta analyzer was performed. The UV–Vis diffuse reflectance spectroscopy (DRS) of the samples was recorded using a Scinco spectrophotometer (S-4100).
3
FTIR Analysis of Purified Fraction
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1 mg of dried purified fraction was ground with 100 mg KBr powder and pressed into pellets to analyze the functional groups using a Fourier transform infrared spectrophotometer (Thermo Fisher, USA) over the frequency range 400 to 4,000 cm -1 .
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