Log In Sign up
The largest database of trusted experimental protocols

Anti h3k9ac 61251

Manufactured by Active Motif
Visit Supplier

Anti-H3K9ac (61251) is a highly specific antibody that recognizes acetylated histone H3 at lysine 9. It is designed for use in various applications, such as chromatin immunoprecipitation (ChIP), western blotting, and immunofluorescence experiments.

Automatically generated - may contain errors

Lab products found in correlation

Gateway technique, by Thermo Fisher Scientific (2 mentions) Anti acetyl histone h4 antibody, by Merck Group (2 mentions) Anti h3k18ac 39755, by Active Motif (2 mentions) Anti gst sc 459 antibody, by Santa Cruz Biotechnology (2 mentions) Fluorescent secondary antibodies 926 32211, by LI COR (2 mentions) Ab7311, by Abcam (2 mentions) Anti flag m2 antibody f1804, by Merck Group (2 mentions) Pgex 6p 1 vector, by GE Healthcare (2 mentions)

Spelling variants of this product

H3K9ac (17 citations) Anti-H3K9ac (12 citations)

2 protocols using anti h3k9ac 61251

1

Cloning and Characterization of p300 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
The coding DNA sequences (CDS) encoding human full-length p300 protein and the p300 BD-RING-PHD-HAT-ZZ-TAZ2 region (BRPHZT, amino acids 1035–1830) were cloned into pENTR3C vector and subsequently cloned into p3FLAG and pCDH-FLAG destination vectors using Gateway techniques (Invitrogen), respectively. The CDS encoding the human p300 BD-RING-PHD-HAT-ZZ region (BRPHZ, amino acids 1035–1720) and ZZ domain (amino acids 1650–1720) were cloned into the pGEX-6P-1 vector (GE Healthcare). Point mutations and deletions were generated using a site-directed mutagenesis kit (Stratagene) and verified by Sanger sequencing. Histone peptides bearing different modifications were synthesized at CPC, LLC. Anti-histone antibodies including anti-H3 (Ab1791), anti-H3K4ac (ab176799), anti-H3K9ac (Ab32129), anti-H3K27ac (Ab4729) and anti-H4 (Ab7311) antibodies were obtained from Abcam. Anti-H3K9ac (61251) and anti-H3K18ac (39755) antibodies were from Active Motif. Anti-acetyl-Histone H4 antibody (06–598) was from Millipore. Anti-GST (sc-459) antibody was from Santa Cruz. Anti-FLAG (M2, F1804) antibody was from Sigma. Fluorescent secondary antibodies (926–32211 and 926–68020) were from LI-COR. Mononucleosomes reconstituted from recombinant histones (16–0009) were from EpiCypher.
Zhang Y., Xue Y., Shi J., Ahn J., Mi W., Ali M., Wang X., Klein B.J., Wen H., Li W., Shi X, & Kutateladze T.G. (2018). The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3. Nature structural & molecular biology, 25(9), 841-849.
+ Open protocol
+ Expand
2

Cloning and Characterization of p300 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
The coding DNA sequences (CDS) encoding human full-length p300 protein and the p300 BD-RING-PHD-HAT-ZZ-TAZ2 region (BRPHZT, amino acids 1035–1830) were cloned into pENTR3C vector and subsequently cloned into p3FLAG and pCDH-FLAG destination vectors using Gateway techniques (Invitrogen), respectively. The CDS encoding the human p300 BD-RING-PHD-HAT-ZZ region (BRPHZ, amino acids 1035–1720) and ZZ domain (amino acids 1650–1720) were cloned into the pGEX-6P-1 vector (GE Healthcare). Point mutations and deletions were generated using a site-directed mutagenesis kit (Stratagene) and verified by Sanger sequencing. Histone peptides bearing different modifications were synthesized at CPC, LLC. Anti-histone antibodies including anti-H3 (Ab1791), anti-H3K4ac (ab176799), anti-H3K9ac (Ab32129), anti-H3K27ac (Ab4729) and anti-H4 (Ab7311) antibodies were obtained from Abcam. Anti-H3K9ac (61251) and anti-H3K18ac (39755) antibodies were from Active Motif. Anti-acetyl-Histone H4 antibody (06–598) was from Millipore. Anti-GST (sc-459) antibody was from Santa Cruz. Anti-FLAG (M2, F1804) antibody was from Sigma. Fluorescent secondary antibodies (926–32211 and 926–68020) were from LI-COR. Mononucleosomes reconstituted from recombinant histones (16–0009) were from EpiCypher.
Zhang Y., Xue Y., Shi J., Ahn J., Mi W., Ali M., Wang X., Klein B.J., Wen H., Li W., Shi X, & Kutateladze T.G. (2018). The ZZ domain of p300 mediates specificity of the adjacent HAT domain for histone H3. Nature structural & molecular biology, 25(9), 841-849.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!