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Nc1029572

Manufactured by Thermo Fisher Scientific
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The NC1029572 is a laboratory pipette from Thermo Fisher Scientific. It is designed for precise liquid handling and transfer in various laboratory applications. The pipette features an ergonomic design and adjustable volume settings to accommodate a wide range of liquid volumes.

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Lab products found in correlation

Ht501128, by Merck Group (3 mentions) F6057, by Merck Group (3 mentions)

3 protocols using nc1029572

1

Rat Heart Tissue Preparation

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Rat hearts were excised under anesthesia after collection of echocardiographic data and perfused with 10% formalin solution, (Sigma Aldrich #HT501128). Tissues were cryopreserved using 30% sucrose (prepared in 1xPBS) and embedded in OCT (Fisher Scientific, TissueTek #NC1029572). Sections were cut to 7 μm using a commercial cryostat and stained for different antibodies according to manufacturer’s instruction. Cells and tissue sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Sigma #F6057).
Gunasekaran M., Mishra R., Saha P., Morales D., Cheng W.C., Jayaraman A.R., Hoffman J.R., Davidson L., Chen L., Shah A.M., Bittle G., Fu X., Tulshyan A., Abdullah M., Kingsbury T., Civin C., Yang P., Davis M.E., Bolli R., Hare J.M., Sharma S, & Kaushal S. (2022). Comparative efficacy and mechanism of action of cardiac progenitor cells after cardiac injury. iScience, 25(8), 104656.
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2

Histological Analysis of Rat Hearts

Cited 1 time
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Tissues were processed as described previously [8 (link)]. Briefly, rat hearts were excised under anesthesia after collection of echocardiographic data and perfused with 10% formalin solution, (Sigma Aldrich #HT501128). Tissues were cryopreserved using 30% sucrose (prepared in 1× PBS) and embedded in optimal cutting temperature compound (Fisher Scientific, TissueTek #NC1029572). A commercial cryostat was used to cut 7-μm sections, which were stained for different antibodies according to the manufacturers’ instructions. Tissue sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) nuclear stain (Sigma #F6057) together with other required stains such as Foxp3 and pP65. All images were obtained with an EVOS microscope and quantified using Image J software.
Mishra R., Saha P., Datla S.R., Mellacheruvu P., Gunasekaran M., Guru S.A., Fu X., Chen L., Bolli R., Sharma S, & Kaushal S. (2022). Transplanted allogeneic cardiac progenitor cells secrete GDF-15 and stimulate an active immune remodeling process in the ischemic myocardium. Journal of Translational Medicine, 20, 323.
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3

Cryosectioning and Immunostaining of Rat Hearts

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Tissues were processed as described previously [4] . Briefly, after collection of echocardiographic data, rat hearts were excised under anesthesia and perfused with 10% formalin (Sigma Aldrich #HT501128). Tissues were cryopreserved in 30% sucrose in 1× PBS and embedded in optimal cutting temperature compound (Fisher Scientific, TissueTek #NC1029572). Sections (7 μm) were cut with a commercial cryostat, stained with different antibodies according to the manufacturers' instructions, and counterstained with 4′,6-diamidino-2-phenylindole (Sigma #F6057) together with other required stains such as mCherry/CD44. All images (>5/slide) were obtained with an EVOS microscope and quantified using Image J software.
Saha P., Guru S.A., Ge Z.D., Simms L., Chen L., Bolli R, & Kaushal S. (2024). Neonatal Cardiac Mesenchymal Stromal Cells Promote Recovery of Infarcted Myocardium through CD44 Mediated FoxP3+ T-Regulatory Cells after Vascular Infusion. Stem cell reviews and reports.
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