22 hydroxycholesterol

Manufactured by Merck Group

Sourced in United States

22-hydroxycholesterol is a chemical compound that is a derivative of cholesterol. It serves as a precursor for the synthesis of other important biological molecules. This compound is primarily used in research applications involving the study of cholesterol metabolism and related physiological processes.

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Lab products found in correlation

Pregnenolone, by Steraloids (1 mentions) Mouse monoclonal anti β actin antibody, by Merck Group (1 mentions) Dibutyryl camp db camp, by Merck Group (1 mentions) Pregnenolone, by Merck Group (1 mentions) Trypan blue stain, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Sybr green pcr reagent, by Thermo Fisher Scientific (1 mentions) Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions) Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Collagenase type 1, by Worthington (1 mentions)

2 protocols using 22 hydroxycholesterol

Steroidogenic Enzyme Regulation in Cells

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Dibutyryl cAMP (dbcAMP) and 22-hydroxycholesterol were obtained from Sigma-Aldrich. Pregnenolone was obtained from Steraloids (purity 99.8%). Testosterone antibody was obtained from Fitzgerald Industries International. Bovine LH (USDA-bLH-B-6) was provided by the United States Department of Agriculture Animal Hormone Program [16 (link)].
Polyclonal rabbit anti-cytochrome P450scc cholesterol side-chain cleavage enzyme (P450scc) antibody was obtained from Millipore (#ABS235). Polyclonal rabbit anti-steroidogenic acute regulatory protein (StAR) antibody was obtained from Santa Cruz Biotechnology (#25806). Monoclonal mouse anti-gp91^phox antibody was obtained from BD Transduction Laboratories (#611414). Polyclonal rabbit anti-4-hydroxy-2-nonenal (HNE) antibody was obtained from Alpha Diagnostic International (#HNE-11S). Polyclonal rabbit anti-glutathione peroxidase-1 (GPx-1) antibody was obtained from Abcam (#22604). Monoclonal mouse anti β-actin antibody was obtained from Sigma Chemical (#A5316). The horseradish peroxidase-conjugated anti-mouse (#NA931V) or anti-rabbit (#NA934V) donkey second antibodies were obtained from GE Healthcare Biosciences.

Musicki B., Zhang Y., Chen H., Brown T.R., Zirkin B.R, & Burnett A.L. (2015). Mechanism of Testosterone Deficiency in the Transgenic Sickle Cell Mouse. PLoS ONE, 10(5), e0128694.

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Isolation and Culture of Ovarian Cells

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Cell culture Medium 199, trypsin-EDTA (0.25%), penicillin G, streptomycin sulfate, fetal bovine serum, Hank's balanced salt solution, and trypan blue stain were purchased from Life Technologies (Grand Island, NY, USA). Collagenase-type 1 was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA). Bovine serum albumin, 22-hydroxycholesterol, pregnenolone, and other general chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Kisspeptin10 and its antagonist (peptide234) were synthesized by Kelowna International Scientific, Inc. (Taipei, Taiwan), and ovine LH was obtained from the National Institute of Diabetes and Digestive and Kidney Diseases. All nucleotide primers were obtained from Bio Basic, Inc. (Markham, Canada). Reverse-transcription kits were purchased from Bioline (London, UK). SYBR-Green PCR reagent for semiquantitative real-time PCR was also purchased from Life Technologies.

Wang J.Y., Hsu M.C., Tseng T.H., Wu L.S., Yang K.T, & Chiu C.H. (2015). Kisspeptin expression in mouse Leydig cells correlates with age. Journal of the Chinese Medical Association : JCMA, 78(4).

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