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Pcr buffer solutions

Manufactured by Biofact

PCR buffer solutions are essential components for polymerase chain reaction (PCR) processes. They provide the necessary ionic environment and pH conditions to facilitate the enzymatic amplification of DNA sequences. These solutions contain a balanced mixture of salts, buffers, and other additives to ensure optimal performance of the PCR reaction.

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2 protocols using pcr buffer solutions

1

Surface Functionalization of Polymers

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(3-Aminopropyl)triethoxysilane (APTES; 99%) and [2-(3,4-epoxycyclohexyl)ethyl]trimethoxysilane (ECTMS; 98%) were purchased from Sigma-Aldrich. The PDMS prepolymer (Sylgard 184) and curing agent were obtained from Dow Corning. Poly(methyl methacrylate) (PMMA), polycarbonate (PC), polystyrene (PS), and polyethylene terephthalate (PET) were purchased from Goodfellow. Fluospheres amine (0.2 μm, red) and TE buffer (10 nM Tris–HCl, 0.1 nM EDTA, pH 8.0) were obtained from Thermo Fisher Scientific. Taq polymerase, PCR buffer solutions, and dNTPs were obtained from BioFact (Daejeon, Korea). Agarose powder was purchased from BioShop (Ontario, Canada). A 100 bp DNA size marker was purchased from Takara (Shiga, Japan). Ethidium bromide (EtBr)-based dye (Loading STAR) was purchased from Dynebio (Seongnam, Korea).
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2

TwistAmp Basic Kit Protocol

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TwistAmp Basic kits were purchased from TwistDx (Cambridge, UK). Poly(dimethylsiloxane) (PDMS) pre-polymer (Sylgard 184) and curing agent were purchased from Dow Corning (Midland, MI, USA). Taq DNA polymerase, PCR buffer solutions, and dNTPs were purchased from BioFact (Daejeon, Korea). Primer pairs were purchased from Bioneer (Daejeon, Korea). The DNA ladder (100 bp) was purchased from Takara (Shiga, Japan) and agarose powder was purchased from BioShop (Ontaria, Canada). Loading Star was purchased from DYNE Bio (Seongnam, Korea). SYBR Green I (10,000×) was purchased from Lonza (Basel, Switzerland). Luria Bertani (LB) broth (with low salt) and agar powder (bacteriological) were purchased from MB cells (Seoul, Korea).
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