4 chloromethcathinone

4-chloromethcathinone (Clephedrone, 4-CMC), N-ethyl Pentedrone (α-Ethylaminopentiophenone, NEP), N-ethyl Hexedrone (α-Ethylaminohexanophenone, NEH), and Methylone-d₃ (deuterated internal standard, IS) were purchased from Cayman (Ann Arbor, MI, USA). Pentafluoropropionic anhydride (PFPA) was supplied from Merk Life Science SRL (Milano, Italy).
LC-MS/MS grade water, methanol, acetonitrile, LC-MS grade formic acid and ethyl acetate, ammonium hydrogen carbonate and sodium hydroxide were purchased from Carlo Erba (Cornaredo, Italy).

Synthetic β-cathinones: 3-chloromethcathinone [3-CMC, 1-(3-chlorophenyl)-2-(methylamino)-1-propanone], 4-chloromethcathinone [4-CMC, 1-(4-chlorophenyl)-2-(methylamino)-1-propanone], 4-methoxy-α-pyrrolidinopentiophenone [4-MeO-PVP, 1-(4-methoxyphenyl)-2-(1-pyrrolidinyl)-1-pentanone], and 4-fluoro-α-pyrrolidinopentiophenone [4-F-PVP, 1-(4-fluorophenyl)-2-(1-pyrrolidinyl)-1-pentanone] were purchased in the form of their hydrochloride salts from Cayman Chemical (Ann Arbor, MI, USA). Isotonic solution of saline for injections (0.9% NaCl) was purchased from Polska Grupa Farmaceutyczna (Łódź, Poland). Cell culture reagents: Dulbecco’s modified Eagle’s medium with F12 supplement (DMEM/F12), heat-inactivated fetal bovine serum (FBS), phosphate-buffered saline (PBS), Trypsin-EDTA, penicillin, streptomycin, and amphotericin B were purchased from Life Technologies (Warsaw, Poland). Dimethyl sulfoxide (DMSO), Triton X-100, and MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) were purchased from Sigma-Aldrich (Poznań, Poland).