All of the kimchi samples were lyophilizated and freeze-dried kimchi samples underwent an extraction process with 20 times of methanol by stirring overnight. Finally, the kimchi methanol extracts were concentrated by heat evaporation (Eyela rotary evaporator system) and stored at 4°C. Since the usual serving dose of kimchi in Korea is approximately 100 g/day upon individual taste, the extracted cpkimchi was mixed into diet pellet, changed daily, in two serving dose for animal, 1.7 g/kg/day and 5.1 g/kg/day equivalent with usual general intake dose of kimchi in Korean. The skimchi, cpkimchi and kimuchi extracts for in vitro experiment was dissolved into 5 mg/ml and 10 mg/ml in order to execute in vitro-cell experiment.
Rotary evaporator system
Manufactured by Eyela
Sourced in Japan
The Rotary Evaporator System is a lab equipment designed to efficiently remove solvents from liquid samples through the process of evaporation. It consists of a rotating flask that is immersed in a heated water bath, allowing for controlled and gentle solvent removal under vacuum conditions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rotary evaporator system
1
Lyophilized Kimchi Extraction and In Vitro Evaluation
2
HPLC Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Evaporation was conducted using an Eyela rotary evaporator system (Tokyo, Japan) under reflux in vacuo. HPLC chromatograms were recorded with an Agilent 1100 series HPLC (Waldbronn, Germany) equipped with Agilent 1100 series G1311A Quat pump and Agilent 1100 series G1315B detector. A Discovery C18 (4.6 × 250 mm, 5 μm) column was purchased from Sigma-Aldrich Co. (PA, USA). Water and acetonitrile used in this research were of HPLC grade, and all other reagents were of analytical grade.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!