PMN-MDSCs were separated by magnetic beads (BioLegend), and CD8+ T-cells were separated by CD8+ T Cell Enrichment Kit (BioLegend). Then, PMN-MDSCs and carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) labeled CD8+T cells were cocultured for 72 h. To count the CD8+CFSE+ and CD8+IFN-γ+ T-cell subpopulation, T-cells were first stained with FITC-labeled anti-CD8 (Biolegend), and then stained with PE-labeled anti-IFN-γ antibody (Biolegend). The CD8+CFSE+ and CD8+IFN-γ+ T-cells were detected by flow cytometry (Becton Dickinson).
Pe labeled anti ifn γ antibody
Manufactured by BioLegend
The PE-labeled anti-IFN-γ antibody is a fluorescently-conjugated monoclonal antibody that specifically binds to the interferon-gamma (IFN-γ) protein. The PE (Phycoerythrin) fluorescent label allows for the detection and quantification of IFN-γ-producing cells using flow cytometry.
Automatically generated - may contain errors
2 protocols using pe labeled anti ifn γ antibody
1
Quantification of CD8+ T cell suppression by PMN-MDSCs
2
Suppression of CD8+ T Cell Proliferation by PMN-MDSCs
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CD8 + T cells were isolated from the spleen of C57BL/6 mice by CD8 + T Cell Enrichment Kit (BioLegend). PMN-MDSCs were isolated from the tumor or spleen of C57BL/6 mice by magnetic beads (BioLegend). PMN-MDSCs were then cocultured with CFDA-SE (Carboxy uorescein diacetate, succinimidyl ester) labeled CD8 + T cells in the medium containing anti-CD3 (1 µg/mL) and anti-CD28 (1 µg/mL) antibodies (BD Biosciences) for 72 hours. To enumerate the CFSE + CD8 + and IFN-γ + CD8 + T-cell subset, cells were rst stained with FITC-labeled anti-mCD8 (Biolegend), then xed or permeabilized before they were stained with PE-labeled anti-IFN-γ antibody (Biolegend). The CFSE + CD8 + and IFN-γ + CD8 + T cells were detected by ow cytometry (Becton Dickinson, San Jose, CA, USA).
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