EYPC was kindly donated by NOF Co. (Tokyo, Japan). 1,2-Cyclohexanedicarboxylic anhydride, OVA, fluorescein isothiocyanate (FITC), p-xylene-bis-pyridinium bromide (DPX) were purchased from Sigma (St. Louis, MO, USA). Dextran having molecular weight of 70,000, 1-aminodecane, pyranine and Triton X-100 were obtained from Tokyo Chemical Industries Ltd. (Tokyo, Japan). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride (DMT-MM) was from Wako Pure Chemical Industries Ltd. (Osaka, Japan). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) was from Life Technologies. Dialysis tube (molecular weight cutoff: 10 kDa) was purchased from Sekisui Medical Co., Ltd. (Tokyo, Japan). FITC-OVA was prepared by reacting OVA (10 mg) with FITC (11.8 mg) in 0.5 M NaHCO3 (4 mL, pH 9.0) at 4 °C for three days and subsequent dialysis [13 (link)]. 3-Methyl glutarylated dextrans (MGlu65-Dex and MGlu67-Dex-C10) were prepared as previously reported [15 (link)].
Pyranine
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Pyranine is a fluorescent dye used in various analytical and scientific applications. It is a water-soluble compound that exhibits strong fluorescence properties, making it a useful tool for various measurements and detection methods in the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
1 aminodecane, by Tokyo Chemical Industry (2 mentions)
Triton x 100, by Tokyo Chemical Industry (2 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (2 mentions)
Ovalbumin (ova), by Merck Group (2 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (2 mentions)
4 4 6 dimethoxy 1 3 5 triazin 2 yl 4 methylmorpholinium chloride dmt mm, by Fujifilm (1 mentions)
P xylene bis pyridinium bromide dpx, by Merck Group (1 mentions)
Dextran sulfate sodium salt, by Merck Group (1 mentions)
4 4 6 dimethoxy 1 3 5 triazin 2 yl 4 methyl morpholinium chloride, by Fujifilm (1 mentions)
2 protocols using pyranine
1
Fluorescent Labeling of Ovalbumin
2
pH-Sensitive Liposomes for Targeted Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Saccharomyces cerevisiae for synthesis of pH-sensitive polymers, OVA, MPLA, fluorescein isothiocyanate (FITC), p-Xylene-bis-pyridinium bromide (DPX) for preparation of liposomes, and dextran sulfate sodium salt from Leuconostoc spp. for the evaluation of cellular association of liposomes, were purchased from Sigma (St. Louis, MO.). 1-Aminodecane for synthesis of pH-sensitive polymers, pyranine and Triton X-100 for preparation of liposomes were obtained from Tokyo Chemical Industries Ltd.
(Tokyo, Japan). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride (DMT-MM) was from Wako Pure Chemical Industries Ltd. (Osaka, Japan) for synthesis of pH-sensitive polymers. 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was from Life Technologies for fluorescence labeling of liposomal membrane. FITC-OVA was prepared by reacting OVA (10 mg) with FITC (11.8 mg) in 0.5 M NaHCO 3 (4 mL, pH 9.0) at 4 °C for three days and subsequent dialysis [23] .
pH-sensitive dextran derivatives (MGlu-Dex) were prepared as previously reported [24] .
(Tokyo, Japan). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methyl morpholinium chloride (DMT-MM) was from Wako Pure Chemical Industries Ltd. (Osaka, Japan) for synthesis of pH-sensitive polymers. 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was from Life Technologies for fluorescence labeling of liposomal membrane. FITC-OVA was prepared by reacting OVA (10 mg) with FITC (11.8 mg) in 0.5 M NaHCO 3 (4 mL, pH 9.0) at 4 °C for three days and subsequent dialysis [23] .
pH-sensitive dextran derivatives (MGlu-Dex) were prepared as previously reported [24] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!