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Krg ca 2 containing cell permeable luminol

Manufactured by Merck Group
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KRG-Ca 2+ containing cell permeable luminol is a laboratory equipment product. It is a compound that can be used to detect and measure calcium ion (Ca2+) levels within cells. The core function of this product is to serve as a tool for cellular calcium detection and analysis.

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Lab products found in correlation

Isoluminol, by Merck Group (2 mentions) Superoxide dismutase, by Solarbio (2 mentions) Synergy h1 multi mode reader, by Agilent Technologies (2 mentions) Luminol, by Agilent Technologies (2 mentions) Isoluminol, by Agilent Technologies (2 mentions) Wkymvm peptide, by Merck Group (2 mentions) Formyl methionyl leucyl phenylalanine (fmlp), by Merck Group (2 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (2 mentions)

2 protocols using krg ca 2 containing cell permeable luminol

1

Measuring NADPH Oxidase Activity via Chemiluminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
NADPH oxidase activity was measured by luminol/isoluminol-enhanced chemiluminescence (CL) using a microplate reader (Synergy H1 MultiMode Reader, BioTek, U.S.A) [30] . For intracellular ROS measurement, granulocytes were added to KRG-Ca 2+ containing cell permeable luminol (10 µg/mL; Sigma-Aldrich) and 50 U/mL superoxide dismutase (Solarbio) to scavenge extracellular superoxide, while extracellular ROS was measured in the presence of 10 µg/mL isoluminol (Sigma-Aldrich). Neutrophils and reagents were then incubated for 5 min at 37°C before stimulation with fMLP (1 µM; Sigma-Aldrich), WKYMVM peptide (1 µM; Sigma-Aldrich), or phorbol 12-myristate 13-acetate (PMA) (500 nM; Sigma-Aldrich). CL was recorded continuously for 20-25 min.
Zhang L., Chen Z., Li W., Liu Q., Wang Y., Chen X., Tian Z., Yang Q., An Y., Zhang Z., Mao H., Tang X., Lv G, & Zhao X. (2022). Combined Immunodeficiency Caused by a Novel De Novo Gain-of-Function RAC2 Mutation. Journal of clinical immunology, 42(6).
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2

Measuring NADPH Oxidase Activity via Chemiluminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
NADPH oxidase activity was measured by luminol/isoluminol-enhanced chemiluminescence (CL) using a microplate reader (Synergy H1 MultiMode Reader, BioTek, U.S.A) [30] . For intracellular ROS measurement, granulocytes were added to KRG-Ca 2+ containing cell permeable luminol (10 µg/mL; Sigma-Aldrich) and 50 U/mL superoxide dismutase (Solarbio) to scavenge extracellular superoxide, while extracellular ROS was measured in the presence of 10 µg/mL isoluminol (Sigma-Aldrich). Neutrophils and reagents were then incubated for 5 min at 37°C before stimulation with fMLP (1 µM; Sigma-Aldrich), WKYMVM peptide (1 µM; Sigma-Aldrich), or phorbol 12-myristate 13-acetate (PMA) (500 nM; Sigma-Aldrich). CL was recorded continuously for 20-25 min.
Zhao X., Zhang L., Chen Z., Li W., Liu Q., Wang Y., Chen X., Tian Z., Yang Q., An Y., Zhang Z., Mao H., Tang X., & Lv G. (2022). Combined Immunodeficiency Caused by a Novel De Novo Gain-of-Function RAC2 Mutation.
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