Escherichia coli xl1 blue

Escherichia coli XL1 Blue [recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacI^qZ∆M15 Tn10 (Tet^r)]], obtained from Invitrogen, was used for cloning experiments and plasmid isolation. Luria–Bertani (LB—Sigma, USA) supplemented with 100 mg L⁻¹ ampicillin, 50 mg L⁻¹ chloramphenicol or 50 mg L⁻¹ kanamycin was used as a growth medium.
The wild-type strain, A. adeninivorans LS3, originally isolated from wood hydrolysate in Siberia and deposited as A. adeninivorans SBUG 724 in the strain collection of the Department of Biology of the University of Greifswald [25 (link)] was used as a control strain. The auxotrophic mutant, A. adeninivorans G1216 [aleu2 ALEU2::aade2] [26 (link)] and double auxotrophic mutant, MS1006 [aleu2 atrp1::ALEU2 aade2::ALEU2] [27 (link)] were used as recipient strains. All strains were cultivated at 30 °C, 180 rpm in 50 mL of broth in a 100 mL flask. The medium was either a selective yeast minimal medium supplemented with 20 g L⁻¹ glucose and 43 mM NaNO₃ (YMM-glc-NO₃) [28 , 29 ] or a non-selective yeast complex medium containing 20 g L⁻¹ glucose (YPD).