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Anti his and protein a g plus agarose beads

Manufactured by Santa Cruz Biotechnology
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Anti-His and Protein A/G PLUS-Agarose beads are lab equipment used for the purification and detection of proteins. Anti-His beads are used to capture and purify proteins with a histidine tag, while Protein A/G PLUS-Agarose beads are used to purify antibodies and proteins that bind to the Protein A/G complex.

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Lab products found in correlation

Close spelling variants of this product

Protein A/G PLUS-Agarose Protein G plus/protein A‐agarose Protein A and G beads Protein A/G agarose Protein G PLUS-Agarose Protein A/G PLUS Agarose A/G Protein G-agarose Protein A agarose G/A beads

2 protocols using anti his and protein a g plus agarose beads

1

Fyn Protein Biotinylation and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 24 h after transfection with pcDNA4His-Fyn, HEK293T cells were disrupted using lysis buffer [10 mM Tris (pH 7.5), 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM dithiothreitol (DTT), 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 10% glycerol, and a protease inhibitor cocktail tablet] containing 100 µM BIAM (Invitrogen). Fyn was immunoprecipitated with anti-His and Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology). The immunocomplexes were separated by SDS–PAGE and BIAM labels were detected with HRP-conjugated streptavidin and ECL41 .
Kim J.E., Roh E., Lee M.H., Yu D.H., Kim D.J., Lim T.G., Jung S.K., Peng C., Cho Y.Y., Dickinson S., Alberts D., Bowden G.T., Einspahr J., Stratton S.P., Curiel C., Bode A.M., Lee K.W, & Dong Z. (2015). Fyn is a redox sensor involved in solar ultraviolet light-induced signal transduction in skin carcinogenesis. Oncogene, 35(31), 4091-4101.
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2

Fyn Protein Biotinylation and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 24 h after transfection with pcDNA4His-Fyn, HEK293T cells were disrupted using lysis buffer [10 mM Tris (pH 7.5), 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM dithiothreitol (DTT), 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 10% glycerol, and a protease inhibitor cocktail tablet] containing 100 µM BIAM (Invitrogen). Fyn was immunoprecipitated with anti-His and Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology). The immunocomplexes were separated by SDS–PAGE and BIAM labels were detected with HRP-conjugated streptavidin and ECL41 .
Kim J.E., Roh E., Lee M.H., Yu D.H., Kim D.J., Lim T.G., Jung S.K., Peng C., Cho Y.Y., Dickinson S., Alberts D., Bowden G.T., Einspahr J., Stratton S.P., Curiel C., Bode A.M., Lee K.W, & Dong Z. (2015). Fyn is a redox sensor involved in solar ultraviolet light-induced signal transduction in skin carcinogenesis. Oncogene, 35(31), 4091-4101.
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