Ma5 27896

Manufactured by Thermo Fisher Scientific

The MA5-27896 is a laboratory instrument designed for conducting scientific analyses. It is a versatile and reliable equipment suitable for a variety of applications. The core function of the MA5-27896 is to perform precise measurements and data collection to support research and development activities in various fields.

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Lab products found in correlation

Pd l1, by Thermo Fisher Scientific (2 mentions) Siglec 15, by Affinity Biosciences (2 mentions) Ma5 14548, by Thermo Fisher Scientific (2 mentions) Ab133616, by Abcam (2 mentions) Ab27988, by Abcam (1 mentions)

2 protocols using ma5 27896

Multiplex Immunofluorescence Staining Protocol

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First Panel 1. Siglec-15 (BF8008, Affinity Bioscience, 1:500)—OPAL 520; 2. PD-L1 (MA5-27896, Invitrogen, 1:250)—OPAL 570; 3. CD68 (14-0688-82, Invitrogen, 1:250)—OPAL 620; 4. Pan-cytokeratin (CK) (ab27988, abcam, 1:200)—OPAL 650; 5. DAPI.
Second Panel 1. CD4 (ab133616, abcam, 1:500)—OPAL 480; 2. CD8 (MA5-14548, Invitrogen, 1:350)—OPAL 620; 3. FoxP3 (14-4776-82, Invitrogen, 1:100)—OPAL 650; 4. Siglec-15 (BF8008, Affinity Bioscience, 1:500)—OPAL 520; 5. PD-L1 (MA5-27896, Invitrogen, 1:250)—OPAL 570; 6. CD68 (14-0688-82, Invitrogen, 1:250)—OPAL 780; 7. Pan-CK (ab27988, abcam, 1:200)—OPAL 690; 8. DAPI.

Huang Z., Guo Y., Li B., Shen M., Yi Y., Li L., Zhao X, & Yang L. (2023). Siglec-15 on macrophages suppress the immune microenvironment in patients with PD-L1 negative non-metastasis lung adenocarcinoma. Cancer Gene Therapy, 31(3), 427-438.

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Immunophenotyping of NSCLC Tumor Microenvironment

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IHC was performed on NSCLC whole tissue sections, which were assigned by experienced pathologists. The following primary antibodies were used: CD8 (MA5-14548, Invitrogen), CD4 (ab133616, abcam), FoxP3 (14-4776-82, Invitrogen), Siglec-15 (BF8008, Affinity Bioscience), PD-L1 (MA5-27896, Invitrogen) and CD68 (14-0688-82, Invitrogen).

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