The primary antibodies, p-p53, p-chk2, chk2, γ-h2ax, p-γ-h2ax, monoclonal rabbit antibodies, P53 and GAPDH mouse antibodies were got from cell signalling technology (Danvers, MA). Rabbit anti-human antibodies against p21, Bax, bcl2, cyclin B, and caspase 3 were obtained from proteolytic enzyme technology (Wuhan, China). Secondary antibody was purchased from proteineach technology (Wuhan, China). Red fluorescent antibody, Cy3 goat anti-rabbit and anti-mouse secondary antibody were purchased from Invitrogen (Carlsbad, CA). The matrix gel (356234) was obtained from Corning (Corning, NY).
Gapdh mouse
Manufactured by Cell Signaling Technology
Sourced in United States
GAPDH mouse is a mouse monoclonal antibody that recognizes the Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) protein. GAPDH is a commonly used reference protein for Western blot analysis and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti rabbit cy3, by Thermo Fisher Scientific (1 mentions)
Monoclonal rabbit antibody, by Cell Signaling Technology (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
P chk2, by Cell Signaling Technology (1 mentions)
P γh2ax, by Cell Signaling Technology (1 mentions)
Infinite 200 pro microplate reader, by Tecan (1 mentions)
Clarity ecl substrate, by Bio-Rad (1 mentions)
Blocking solution, by Roche (1 mentions)
Anti mouse sc 2005, by Santa Cruz Biotechnology (1 mentions)
2 protocols using gapdh mouse
1
Protein Expression and Localization
2
Quantification of Aortic Tissue Proteins
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Aortic tissue lysate proteins were quantified by Bradford method in an Infinite 200PRO microplate reader (TECAN, Milan, Italy). Proteins (30 µg) were resolved by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretically blotted onto 0.2 mm nitrocellulose membranes. The membrane were blocked for 1 h at room temperature with 1% blocking solution (Roche) in Tween 20/PBS 0.1% vol/vol (PBST) and then incubated overnight at 4°C with primary antibodies against angiopoietin-1 (goat sc-6319, 1:1000; Santa Cruz, CA, USA), angiopoietin 2 (goat polyclonal sc-7017, 1:1000; Santa Cruz, CA, USA), Tie2 (rabbit sc-9026) and Gapdh (mouse; Cell Signaling Technology, 1:2000). After three washes, the appropriate secondary IgG-HRP-linked conjugate (anti-rabbit A0545; Sigma, anti-mouse sc-2005; Santa Cruz, CA, USA) at 1:3000 dilution was applied. Proteins were visualized with the Clarity ECL substrate (Bio-Rad) and acquired images were quantified using Optiquant software.
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