Samples analyzed by SEC-MALS were buffer-exchanged by dialysis into SEC-MALS running buffer containing 20 mm HEPES (pH 7.6), 200 mm NaCl, 5% glycerol, 0.5 mm TCEP, and 0.05% azide. The samples were applied to a Superose6 10/300 GL (Sigma–Aldrich) column, and in-line measurements were recorded for UV absorbance at 280 nm (using Agilent 1100 series UV detector, Agilent Technologies), static light scattering (DAWN HELEOS 8+light scattering detector, Wyatt Technology), and differential refractive index (Agilent 1200 series refractive index detector, Agilent Technologies) were recorded. System calibration was performed on a BSA standard (Sigma–Aldrich), first resuspended, and then buffer-exchanged into the SEC-MALS running buffer. SEC-MALS data were analyzed with ASTRA version 6.1 software (Wyatt Technology).
Dawn heleos 8 light scattering detector
Manufactured by Wyatt Technology
The DAWN HELEOS 8+ is a multi-angle light scattering detector designed for the analysis of macromolecules and nanoparticles. It measures the intensity of scattered light at multiple angles, providing information about the size, molecular weight, and conformation of the samples.
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Lab products found in correlation
2 protocols using dawn heleos 8 light scattering detector
1
SEC-MALS Analysis of Protein Samples
2
SEC-MALS Analysis of Protein Samples
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Samples analyzed by SEC-MALS were buffer exchanged by dialysis into SEC-MALS running buffer containing 20 mM HEPES (pH 7.6), 200 mM NaCl, 5% glycerol, 0.5mM TCEP, and 0.05% azide. Samples were applied to a Superose6 10/300 GL (Sigma-Aldrich) column and in-line measurements were recorded for ultraviolet absorbance at 280 nm (using Agilent 1100 series UV detector, Agilent Technologies), static light scattering (DAWN HELEOS 8+light scattering detector, Wyatt Technology), and differential refractive index (Agilent 1200 series refractive index detector, Agilent Technologies) were recorded. System calibration was performed on a BSA standard (Sigma-Aldrich), first resuspended, then buffer exchanged into the SEC-MALS running buffer. SEC-MALS data was analyzed with ASTRA v6.1 software (Wyatt Technology).
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