Rat hearts lysates (500 mg protein) were immunoprecipitated for SERCA2 using monoclonal mouse antibody (2 μg/ml, Thermo Scientific) with 20 μl protein G magnetic beads (Cell Signaling Technology). Immunoprecipitated protein captured on the beads was eluted in the sample buffer and resolved on polyacrylamide gels for subsequent Western blotting using SERCA and Br-Tyr antibody as described previously (Ahmad et al. 2019 (link)). Immunoblots on cardiac tissues were performed according to previously described methods using antibodies against SERCA2 (1:1000 abcam), Br-Tyrosine (1:1000; JaICA), phospho-phospholamban (1:1000, Cell Signaling Technology), phospholamban (1:1000, Cell Signaling Technology), PP1 (1:1000 R&D Systems), NOX2 & NOX4 (1:500 Novus Biologicals), GAPDH (1:5000, Cell Signaling Technology), and 4-Hydroxynonenal (1:1000 abcam).
Phospholamban
Manufactured by Cell Signaling Technology
Sourced in United States
Phospholamban is a laboratory reagent used in research applications. It is a small membrane protein that regulates the activity of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) pump, which is responsible for the uptake of calcium into the sarcoplasmic reticulum. Phospholamban plays a crucial role in the regulation of cardiac muscle contractility.
Automatically generated - may contain errors
Lab products found in correlation
Monoclonal mouse antibody, by Thermo Fisher Scientific (1 mentions)
4 hydroxynonenal, by Abcam (1 mentions)
Protein g magnetic beads, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
α smooth muscle actin, by Merck Group (1 mentions)
Phospho camkii, by Cell Signaling Technology (1 mentions)
Rap1a b, by Cell Signaling Technology (1 mentions)
Camkii, by Abcam (1 mentions)
Anti rabbit igg h l hrp conjugated polyclonal antibody, by Stratech Scientific (1 mentions)
2 protocols using phospholamban
1
SERCA2 Immunoprecipitation and Oxidative Modifications
2
Immunoblotting Protocol for Protein Analysis
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For immunoblotting, the primary antibodies used were: phospho‐CaMKII (T286: dilution 1:1000); phospholamban (dilution 1:500), Rap 1A/B (dilution 1:1000) were from Cell Signaling Technology (Beverly, MA, USA). CaMKII (pan: dilution 1:2500) was from Abcam (Cambridge, MA, USA). Smooth muscle α actin (dilution 1:10,000) was from Sigma‐Aldrich. The secondary antibodies used were anti‐mouse IgG (H+L) HRP conjugated polyclonal antibody and anti‐rabbit IgG (H+L) HRP conjugated polyclonal antibody (Stratech Scientific Ltd, Newmarket, UK).
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