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Ab3269p

Manufactured by Merck Group
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The AB3269P is a laboratory equipment product manufactured by Merck Group. It is a device designed to perform a specific function in a laboratory setting. Due to the need to maintain an unbiased and factual approach, a detailed description of the core function of this product cannot be provided. The intended use of this equipment is not something that can be extrapolated or interpreted in this response.

Automatically generated - may contain errors

Lab products found in correlation

Bca kit, by Thermo Fisher Scientific (6 mentions) Ab14041, by Abcam (2 mentions) Ab68418, by Abcam (2 mentions) Ab22595, by Abcam (2 mentions) Ab22759, by Abcam (2 mentions) Ab92726, by Abcam (2 mentions) Ab133625, by Abcam (2 mentions) Itgb1, by Cell Signaling Technology (2 mentions) Sc 376638, by Santa Cruz Biotechnology (2 mentions) Thbs1, by Cell Signaling Technology (2 mentions) Sparc, by Cell Signaling Technology (2 mentions) Ripa lysis buffer, by Merck Group (2 mentions)

2 protocols using ab3269p

1

Quantitative Protein Analysis of sEVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
sEV and cells were resuspended in RIPA lysis buffer (Millipore-Sigma) and incubated on ice for 20 min prior to centrifugation at 12,000 g for 10 min. Supernatants were used for western blotting in SDS-PAGE electrophoresis. Protein concentration was determined by a BCA kit (Thermofisher). The following antibodies were used: ADIPOQ (AB3269P, Millipore-Sigma), CD36 (ab133625, Abcam), HSP90alpha (PA3–013, Thermofisher), POSTN (ab14041, Abcam), PGAM1/4 (sc-376638, Santa Cruz); THBS1 (14778, Cell Signaling), ITGB1 (4706, Cell Signaling), EGFR (2232, Cell Signaling), SPARC (8725, Cell Signaling), IGFBP5 (AF578, R&D systems), CD9 (ab92726, Abcam), CD63 (ab68418, Abcam), CANX (ab22595, Abcam) and FASN (ab22759, Abcam). Given the large difference in the abundance for some of the proteins among different cell types, some of the blots were overexposed to show the signal in as many cell types as possible. Because of this, in some cases, the signal is beyond the linear range, and thus the quantification might not be absolutely accurate. Band intensity quantification was performed using ImageJ software (NIH).
Garcia-Martin R., Brandao B.B., Thomou T., Altindis E, & Ronald Kahn C. (2022). Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism. Cell reports, 38(3), 110277.
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2

Quantitative Protein Analysis of sEVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
sEV and cells were resuspended in RIPA lysis buffer (Millipore-Sigma) and incubated on ice for 20 min prior to centrifugation at 12,000 g for 10 min. Supernatants were used for western blotting in SDS-PAGE electrophoresis. Protein concentration was determined by a BCA kit (Thermofisher). The following antibodies were used: ADIPOQ (AB3269P, Millipore-Sigma), CD36 (ab133625, Abcam), HSP90alpha (PA3–013, Thermofisher), POSTN (ab14041, Abcam), PGAM1/4 (sc-376638, Santa Cruz); THBS1 (14778, Cell Signaling), ITGB1 (4706, Cell Signaling), EGFR (2232, Cell Signaling), SPARC (8725, Cell Signaling), IGFBP5 (AF578, R&D systems), CD9 (ab92726, Abcam), CD63 (ab68418, Abcam), CANX (ab22595, Abcam) and FASN (ab22759, Abcam). Given the large difference in the abundance for some of the proteins among different cell types, some of the blots were overexposed to show the signal in as many cell types as possible. Because of this, in some cases, the signal is beyond the linear range, and thus the quantification might not be absolutely accurate. Band intensity quantification was performed using ImageJ software (NIH).
Garcia-Martin R., Brandao B.B., Thomou T., Altindis E, & Ronald Kahn C. (2022). Tissue differences in the exosomal/small extracellular vesicle proteome and their potential as indicators of altered tissue metabolism. Cell reports, 38(3), 110277.
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