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2 protocols using snu 1

1

Cultivation of Gastric Cell Lines

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GC cell lines (KATO-III, SGC-7901, BGC-823, HGC-27, AGS, NCI-N87, SNU-1 and SNU-16) and normal gastric epithelial cells (GES cells) were bought from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). All the cells were cultured at 37°C in a humidified atmosphere containing 5% of CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% of fetal bovine serum (FBS),100 U/ml penicillin, and 100 µg/ml streptomycin (all from Gibco, Invitrogen Life Technologies, Carlsbad, CA, USA).
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2

Gastric Cancer Tissue Samples and Cell Lines

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Primary GC tissue samples and adjacent normal tissues were obtained from 40 patients who underwent surgical resection at the Second A liated Hospital of Nanchang University. None of the patients had received preoperative chemotherapy, radiotherapy, or other anticancer modalities. After the resection, the tissue samples were immediately frozen in liquid nitrogen and, then, stored in liquid nitrogen until subsequent treatment and analysis. Eight GC cell lines-KATO-III, SGC-7901, BGC-823, HGC-27, AGS, NCI-N87, SNU-1, and SNU-16-as well as normal gastric cells (GES cells) were bought from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). All the cells were cultured at 37°C in a humidi ed atmosphere containing 5% of CO2 in Dulbecco's Modi ed Eagle's Medium (DMEM) supplemented with 10% of fetal bovine serum (FBS),100 U/ml penicillin, and 100 µg/ml streptomycin (all from Gibco, Invitrogen Life Technologies, Carlsbad, CA, USA).
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