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N hydroxysuccinimide

Manufactured by Sisco Research Laboratories
Sourced in India

N-Hydroxysuccinimide is a chemical compound commonly used as an activating agent in various synthetic and analytical applications. It plays a core role in the formation of stable, reactive intermediates that facilitate the coupling of molecules, such as proteins and other biomolecules. This compound serves as a versatile tool in the field of organic synthesis and biochemistry, enabling efficient labeling, conjugation, and modification procedures.

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3 protocols using n hydroxysuccinimide

1

Erlotinib and Doxorubicin Nanoparticle Formulation

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Erlotinib (Mol wt = 393.436, purity of ≥95%) was a gift sample from Natco Pharma Ltd. UPSIDC (Dehradun, India). Doxorubicin also a gift sample from Neon Laboratories Pvt. Ltd. (Ghaziabad, India).The cinnamon biopolymer was purchased from Shree Ram Overseas (New Delhi, India). The polyvinyl alcohol (PVA) was received from Sisco Research Laboratory Pvt. Ltd. (Mumbai, India). The cross-linking agents EDC [1-(3 Dimethylaminopropyl)-3-Ethyl Carbodiimide Hydrochloride] and Sulpho-NHS [N-Hydroxysuccinimide] were received from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). The solvent, Dimethyl Sulfoxide (DMSO), was obtained from Merck Pvt. Ltd. (Mumbai, India), and acetone was obtained from SD Fine Chem Pvt. Ltd. (Mumbai, India), HPLC-grade water and other reagents were used as received.
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2

Graphite-based Immunomodulatory Nanocomposite

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Graphite powder, 30% hydrogen peroxide (H2O2), Nhydroxysuccinimide (NHS), 1-(3-Dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC), sulphuric acid (H2SO4), hydrochloric acid (HCl) and ethanol were obtained from Sisco Research Laboratories Pvt Ltd, (Mumbai, India). Chitosan (CS) was purchased from Marine Hydrocolloid (Kerala), Xyloglucan extracted from Tamarind seed powder was obtained as a gift sample from Indra Agrotech Private Ltd (Maharashtra, India). Brown seaweed Sargassum wightii was collected from Mandapam in the Gulf of Mannar. Fetal bovine serum (FBS), DMEM medium, antibiotics were purchased from HiMedia, India. A human histiocytic lymphoma (U 937) cell was obtained from National Centre for Cell Science (NCCS), Pune, India. All other reagents used in this research were of analytical quality.
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3

Lipid-based Nanoformulation for Cancer Therapy

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Materials DADS and RAGE antibody were purchased from Santa Cruz Biotechnology, India. Palmitic acid was obtained from SD-Fine chemicals limited, Mumbai, India. Acetonitrile used was HPLC grade purchased from Merck, India. Sulforhodamine B (SRB), Poloxamer 188 (F68), Sodium dodecyl sulphate were purchased from Sigma-Aldrich, Bangalore, India. 1-ethyl-3-(3dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS, 98%) was purchased Sisco Research Laboratories Pvt. Ltd, India. AnnexinV-FITC and propidium iodide were procured from Biolegend Europe BV, Netherlands. ER-Tracker TM Green was purchased from Imperial Life Sciences (P) Ltd, Gurgaon, India. Annexin V, Propidium Iodide (PI), Nile red and DAPI (4 0 ,6-Diamidino-2-Phenylindole, Dihydrochloride) from Thermo Fisher Scientific India Pvt Ltd., Bangalore, India.
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