Bzx700 all in one microscopy system

The formalin-fixed lungs were then processed for paraffin-embedding and lung tissue sections were prepared. Histochemical staining with hematoxylin and eosin (H&E) was carried out to assess the gross lung morphology. Serial sections were also stained with Alcian Blue (Richard-Allan Scientific) and Periodic acid Schiff’s reagent stain (AB/PAS) to stain for mucin glycoproteins as described earlier^{17 (link)}. The airway epithelial cell and mucous cell numbers per mm basal lamina^{5 (link)} were measured using the BZX700 All-in-one microscopy system (Keyence Inc., Osaka, Japan). Separate sections were immunostained for airway mucin MUC5AC (Cat# MAB2011; Millipore Inc., MA) and eosinophil (Cat# NBP1-42140; Novus Biologicals, CO) following dual-staining technique as described earlier^{18 (link)}. In all cases, quantification and morphometry was carried out by a person unaware of the slide identity.

SH-SY5Y neuroblastoma cells, human microglia cell line HMC3, HeLa cells, and CHO cells were washed in cold phosphate-buffered saline (PBS), fixed in 4% paraformaldehyde (PFA) in PBS for 10 min, and washed thrice in Tris-buffered saline with. 1% Tween 20 detergent (TBST). Cell permeabilization was carried out using. 4% Triton X-100 for 5 min, followed by blocking with a blocking solution (normal goat serum, 1%; BSA, 3%; gelatin, 1%; Triton X-100, 0.2%; saponin 0.2%) for 30 min, incubation with the IQCK primary antibody overnight, incubation with the Alexa Fluor 568-conjugated anti-rabbit IgG secondary antibody for 1 h, and mounting with 4′,6-diamidino-2-phenylindole (DAPI)-containing Fluormount-G (SouthernBiotech, Birmingham, AL) to visualize the nuclei. Cells positively stained for IQCK and those negative for IQCK were captured in a BZX700 All-in-One microscopy system (Keyence Corp, Itaska, IL, United States) and analyzed.