The concentrations of ADP (Selleckchem, catalog S9368) in macrophages were measured by ADP Assay Kit (MilliporeSigma, catalog MAK133) following the instructions. The process is outlined as follows. A total of 1 × 104 PMAs were directly cultured in the assay microplate and stimulated by LPS or ADP for 24 hours. Working agents were used to lysis cells to release ATP and ADP. In the presence of fluorescein, ATP immediately reacts with the substrate (D-fluorescein) to produce luminescence. ADP was converted to ATP by an enzyme reaction, and this newly formed ATP then reacted with D-luciferin in the previous step. The luminescence (relative light units) was read by a luminometer immediately, using the value obtained from the appropriate standards to plot a standard curve and determine the amount of ADP present in the samples. Mice with colitis in the NC and the ADP groups were all constructed by 3% DSS. The ADP (250 mg/kg) was i.p. injected into the ADP group, while mice from the control group were i.p. injected with the same amount of saline every day for 7 days.
S9368
Manufactured by Merck Group
S9368 is a laboratory instrument designed for the analysis and processing of samples. It is a versatile piece of equipment that can be used in various scientific and research applications.
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Lab products found in correlation
2 protocols using s9368
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Quantifying ADP in Macrophages
2
Quantifying ADP in Macrophages
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The concentrations of ADP (Selleckchem, catalog S9368) in macrophages were measured by ADP Assay Kit (MilliporeSigma, catalog MAK133) following the instructions. The process is outlined as follows. A total of 1 × 104 PMAs were directly cultured in the assay microplate and stimulated by LPS or ADP for 24 hours. Working agents were used to lysis cells to release ATP and ADP. In the presence of fluorescein, ATP immediately reacts with the substrate (D-fluorescein) to produce luminescence. ADP was converted to ATP by an enzyme reaction, and this newly formed ATP then reacted with D-luciferin in the previous step. The luminescence (relative light units) was read by a luminometer immediately, using the value obtained from the appropriate standards to plot a standard curve and determine the amount of ADP present in the samples. Mice with colitis in the NC and the ADP groups were all constructed by 3% DSS. The ADP (250 mg/kg) was i.p. injected into the ADP group, while mice from the control group were i.p. injected with the same amount of saline every day for 7 days.
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