Anti α6 integrin conjugated to pe

Manufactured by BD

Anti-α6-integrin conjugated to PE is a laboratory reagent that consists of an antibody specific for the α6-integrin subunit, conjugated to the fluorescent dye phycoerythrin (PE). This product is designed for use in flow cytometry and other applications that require the detection and identification of cells expressing the α6-integrin subunit.

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Lab products found in correlation

Facsaria cell sorter, by BD (2 mentions) Alexa fluor 700, by BD (1 mentions) Facsdiva software, by BD (1 mentions)

Close spelling variants of this product

Anti-α6-integrin-PE Integrin α6 Anti-α6

2 protocols using anti α6 integrin conjugated to pe

Analysis of Bulge Hair Follicle Stem Cells

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Analysis of bulge, hair follicle stem cells (hfSCs) from adult mouse dorsal back skin was performed as described previously ^{28 (link)}. For hfSCs CD34 marker expression analysis, telogen (P18 Con/Wnt7b cKO; P21, P45, YFP+ Con^Dil/Wnt7b cKO^Dil) HF cell suspensions were stained with the following antibodies, anti-α6-integrin conjugated to PE (1:200; BD Pharminigen) and anti-CD34 coupled to Alexafluor-700 (1:50; BD eBioscience). Cells were gated first for live cells (absence of DAPI incorporation) then for basal hfSCs: α6-integrin^High/CD34^High labeled cell fraction (for the overall hfSCs number in the bulge). For RU486 Dilution Lineage Tracing experiments the basal fraction of bulge hfSCs were analyzed for presence YFP labeled cells. Basal hfSCs: YFP+/α6-integrin^High/CD34^High labeled cell fractions were analyzed with a FACS Aria cell sorter (BD Biosciences equipped with FACS DiVa software).

Kandyba E, & Kobielak K. (2014). Wnt7b is an important intrinsic regulator of hair follicle stem cell homeostasis and hair follicle cycling. Stem cells (Dayton, Ohio), 32(4), 886-901.

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Analyzing Adult Mouse Skin Stem Cells

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Analysis of bulge, hair follicle stem cells (hfSCs) from adult mouse dorsal back skin were performed as described previously ^{6 (link)}. For hfSCs CD34 marker expression analysis, telogen (P59) and anagen (P130) YFP+ Con^RU and dKO^RU cell suspensions were stained with the following antibodies, anti-α6-integrin conjugated to PE (1:200; BD Pharminigen) and anti-CD34 coupled to Alexafluor-700 (1:50; BD eBioscience). Cells were gated first for live cells (absence of DAPI incorporation) and then through the YFP+ channel to specifically examine viable YFP+ hair cell populations. Basal hfSCs: YFP+/α6-integrin^High/CD34^High labeled cell fractions were then analyzed with a FACS Aria cell sorter (BD Biosciences equipped with FACS DiVa software).

Kandyba E., Hazen V.M., Kobielak A., Butler S.J, & Kobielak K. (2014). Smad1&5 but not Smad8 establish stem cell quiescence which is critical to transform the premature hair follicle during morphogenesis towards the postnatal state. Stem cells (Dayton, Ohio), 32(2), 534-547.

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