Methanol, water, acetic acid and formic acid ULC/MS grade were from Biosolve (Valkenswaard, The Netherlands). Ethanol HPLC grade was purchased from Honeywell Riedel‐de Haën (Seelze, Germany). Dextromethorphan in Methanolic solution (1 mg·ml−1) was provided from Lipomed AG (Arlesheim, Switzerland). Dextrorphan tartrate, hydrocodone‐D6 and phenobarbital‐D5 in Methanolic solutions (1 mg·ml−1) were purchased from Cerilliant Corporation (Round Rock, USA). Ammonium acetate, potassium phosphate dibasic, potassium phosphate monobasic, nicotinamide adenine dinucleotide phosphate (NADP), isocitric acid, magnesium chloride hexahydrate and isocitrate dehydrogenase were purchased from Sigma‐Aldrich (Buchs, Switzerland).
Ethanol hplc grade
Manufactured by Honeywell
Sourced in Germany
Ethanol HPLC grade is a high-purity solvent used in high-performance liquid chromatography (HPLC) applications. It is a clear, colorless liquid with a characteristic odor. The product meets the specifications required for HPLC-grade solvents, ensuring consistent performance and reliable results in analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Potassium phosphate dibasic, by Merck Group (1 mentions)
Isocitrate dehydrogenase, by Merck Group (1 mentions)
Isocitric acid, by Merck Group (1 mentions)
Magnesium chloride hexahydrate, by Merck Group (1 mentions)
Phenobarbital d5, by Cerilliant (1 mentions)
Formic acid ulc ms grade, by Biosolve (1 mentions)
Nicotinamide adenine dinucleotide phosphate, by Merck Group (1 mentions)
Hydrocodone d6, by Cerilliant (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Potassium phosphate monobasic, by Merck Group (1 mentions)
Methanol, by Biosolve (1 mentions)
Acetic acid, by Biosolve (1 mentions)
Dextromethorphan, by Lipomed (1 mentions)
Meoh d4, by Merck Group (1 mentions)
Dmso d6, by Merck Group (1 mentions)
2 protocols using ethanol hplc grade
1
Quantitative Liquid Chromatography-Mass Spectrometry Analysis
2
Cereulide Extraction and Quantification
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The following compounds and samples were obtained commercially: dimethyl-sulfoxide (≥99%; DMSO), DMSO-d6, and MeOH-d4 from Sigma-Aldrich (Steinheim, Germany); ethanol (HPLC grade) from Honeywell (Seelze, Germany); 13C6-cereulide (>95%) from Chiralix (Nijmegen, Netherlands); and sunflower oil, cow milk (pasteurized, 0.5% fat content), and cow milk (pasteurized, 3.5% fat content) from a local supermarket.
Cereulide was obtained from a B. cereus culture of the emetic reference strain F4810/72. Cultivation, extraction, and isolation were performed as reported recently [33 (link)]. For all experiments, solutions of cereulide (200 µg/mL; DMSO) and 13C6-cereulide (1 µg/mL; EtOH) were applied. Concentration of cereulide and 13C6-cereulide was determined via q-1H-NMR spectroscopy and diluted accordingly.
Cereulide was obtained from a B. cereus culture of the emetic reference strain F4810/72. Cultivation, extraction, and isolation were performed as reported recently [33 (link)]. For all experiments, solutions of cereulide (200 µg/mL; DMSO) and 13C6-cereulide (1 µg/mL; EtOH) were applied. Concentration of cereulide and 13C6-cereulide was determined via q-1H-NMR spectroscopy and diluted accordingly.
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