The following antibodies were used in the immunoblotting: anti-FTSJ1 (Santa Cruz Biotechnology, SC-390355), anti-Flag (Sigma-Aldrich, F1804), anti-ATF3 (Cell Signaling Technology, 18665S), anti–β-actin (Sigma-Aldrich, A5441), anti-phosphorylated eIF2α (Cell Signaling Technology, 3398P), anti-eIF2α (Cell Signaling Technology, 5324), and anti-puromycin antibody (Developmental Studies Hybridoma Bank, PMY-2A4). Yeast Xrn-1 [New England Biolabs (NEB), M0338], yeast tRNA (Sigma-Aldrich, R4018), the microRNA marker (NEB, N2102S), and the Low Range ssRNA Ladder (NEB, N0364S) were acquired commercially.
Anti puromycin antibody
Manufactured by Developmental Studies Hybridoma Bank
The Anti-puromycin antibody is a laboratory reagent used for the detection and quantification of puromycin, a protein synthesis inhibitor. This antibody can be used in various experimental techniques, such as Western blotting and immunohistochemistry, to identify the presence and distribution of puromycin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Yeast trna, by Merck Group (1 mentions)
Anti atf3, by Cell Signaling Technology (1 mentions)
Microrna marker, by New England Biolabs (1 mentions)
Anti phosphorylated eif2α, by Cell Signaling Technology (1 mentions)
Anti flag, by Merck Group (1 mentions)
Anti eif2α, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
P110α antibody, by Cell Signaling Technology (1 mentions)
Puromycin, by Thermo Fisher Scientific (1 mentions)
Tgx 221, by Selleck Chemicals (1 mentions)
α tubulin antibody t6074, by Merck Group (1 mentions)
Irdye 680rd donkey anti mouse igg, by LI COR (1 mentions)
Irdye 800cw goat anti rabbit igg, by LI COR (1 mentions)
2 protocols using anti puromycin antibody
1
Immunoblotting with Commercial Antibodies
2
Western Blotting Antibodies and Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The phospho-S6 antibody (Ser235/236, #2211), S6 antibody (#2317), and p110α antibody (#4255) were obtained from Cell Signaling Technology. p110δ antibodies (#sc-7176, mab2687) were obtained from Santa Cruz Biotechnology and R&D Biosciences, respectively. The p110β antibody (#09-482) was obtained from EMD Millipore, the α-tubulin antibody (T6074) was obtained from Sigma-Aldrich, and the anti-Puromycin antibody was purchased from the Developmental Studies Hybridoma Bank (University of Iowa). Secondary antibodies for Western blotting were obtained from VWR (horseradish peroxidase-coupled anti-mouse and anti-rabbit antibodies for ECL) or from Li-COR Biosciences (IRDye 680 RD donkey anti-mouse IgG and IRDye 800CW goat anti-rabbit IgG for infrared fluorescent Western blotting). Puromycin was purchased from Life Technologies, the p110δ inhibitor IC87114 was purchased from EMD Millipore, and the p110β inhibitor TGX-221 was purchased from Selleck Chemicals.
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