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Ca301

Manufactured by Yamato Scientific
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Sourced in China

The CA301 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, accommodating a variety of sample tubes and vials. The centrifuge provides reliable and consistent performance for a wide range of sample preparation and separation needs.

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Lab products found in correlation

3 protocols using ca301

1

Extraction of Bioactive Compounds from Allium mongolicum

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Seventy-five percent ethanol (80176961, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) was mixed with Allium mongolicum Regel powder at a ratio of 5:1 (v/w), and the mixture was sonicated in an ultrasonic bath (KQ-300DE, Kunshan Ultrasonic Instruments Co., Ltd., Jiangsu, China) (75 watts, 15 min). Then, the ethyl alcohol was evaporated from the mixture by a rotary evaporator (IKA HB10, IKA India Private Limited, Germany). The concentrate was lyophilized in a freeze-dryer (CA301, Yamato Scientific, China) and then pulverized. Previous studies reported that the Allium mongolicum Regel water extract and ethanol extract, having higher phenolic contents (10.28 and 8.50 mg/g dry weight, respectively) and flavonoid contents (4.52 and 3.92 mg/g dry weight, respectively) [16 (link)].
Liu W, & Ao C. (2020). Effect of dietary supplementation with Allium mongolicum Regel extracts on growth performance, carcass characteristics, and the fat color and flavor-related branched-chain fatty acids concentration in ram lambs. Animal Bioscience, 34(7), 1134-1145.
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2

Extraction and Characterization of Allium mongolicum Leaves

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Dried powder of Allium mongolicum Regel leaves was purchased from Hao Hai Biological Company (Alxa League, Inner Mongolia, China). Allium mongolicum Regel water and ethanol extracts were provided by the Animal Nutrition and Immunology Laboratory of Inner Mongolia Agricultural University. Briefly, Allium mongolicum Regel powder was added to distilled water at a ratio of 1:20 (w/v) and placed in a water bath (HH-8, Guohua Electric Appliance Co., Ltd., Jiangsu, China) set at 80°C for 8 h. Then, the mixture was filtered with a vacuum filter system (SHB-III, Zhengzhou Greatwall Scientific Industrial and Trade Co., Ltd., Henan, China) to obtain the filtrate. After that, the filtrate was concentrated with a rotary evaporator (IKA HB10, IKA India Private Limited, Staufen, Germany) at 78°C and a speed of 60 rpm (model: R1002B, Yamato Scientific, Beijing, China), lyophilized in a freeze-dryer (CA301, Yamato Scientific, China) to remove any remaining water and then pulverized.
Liu W, & Ao C. (2020). Effect of dietary supplementation with Allium mongolicum Regel extracts on growth performance, carcass characteristics, and the fat color and flavor-related branched-chain fatty acids concentration in ram lambs. Animal Bioscience, 34(7), 1134-1145.
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3

Extraction and Identification of Active Compounds from Artemisia mongolicum

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The extraction process is described as follows and is also outlined in Figure.

Decoloration and degreasing procedures were similar to those used for AWE.

Extraction

A. mongolicum Regel decolored powder was mixed with 75% ethyl alcohol (80176961, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) at a ratio of 5:1 (vol/wt), and then the mixture was shaken with an ultrasonic cleaner (KQ-300DE, Kunshan Ultrasonic Instruments Co., Ltd., Jiangsu, China; 75 W, 15 min) and a fluid with residues was obtained.

Filtration

The fluid from the mixture was retained, and the residue was extracted again as described in step 2. The resulting fluid from the first and second extractions was fat-soluble extract fluid (AFEF).

Concentration

Ethyl alcohol in the AFEF was evaporated using a rotary evaporator (IKA HB10, IKA India Private Limited; 78 °C, 60 rpm) and the AFEF was freeze-dried (CA301, Yamato Scientific) to obtain AFE. The same UPLC-ESI-MS/MS system used for AWE was adopted to identify the structures in the active ingredients of AFE according to Li et al. (2019) . The results are shown in Table 1.
Ding H., Liu W., Erdene K., Du H, & Ao C. (2021). Effects of dietary supplementation with Allium mongolicum Regel extracts on growth performance, serum metabolites, immune responses, antioxidant status, and meat quality of lambs. Animal Nutrition, 7(2), 530-538.
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