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Dsrna quantitative kit

Manufactured by Vazyme
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Sourced in China

The DsRNA quantitative kit is a laboratory tool designed to accurately measure the concentration of double-stranded RNA (dsRNA) in a sample. It provides a reliable and efficient method for quantifying dsRNA levels, which is an important step in various research and applications involving RNA interference (RNAi) technology.

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Lab products found in correlation

Pyrophosphatase, by Vazyme (1 mentions) Murine rnase inhibitor, by Vazyme (1 mentions) Site directed mutagenesis kit, by Vazyme (1 mentions)

2 protocols using dsrna quantitative kit

1

T7-based Heterologous Protein Expression

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The pQE-30 plasmid and Escherichia coli BL21(DE3) were purchased from Novagen Inc. (Madison, WI, USA) for T7 RNAP heterologous expression. The site-directed mutagenesis kit and dsRNA quantitative kit were supplied by Vazyme Co., Ltd. (Nanjing, China). ATP, GTP, CTP, UTP, pyrophosphatase, and murine RNase inhibitor were purchased from Vazyme Co., Ltd. (Nanjing, China). MgCl2, glycerol, dithiothreitol (DTT), and other chemicals were purchased from Aladdin Industrial Inc. (Shanghai, China) and other commercial sources.
He W., Zhang X., Zou Y., Li J., Chang L., He Y.C., Jin Q, & Ye J. (2024). Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst. Frontiers in Bioengineering and Biotechnology, 12, 1356354.
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2

Optimizing circRNA Synthesis Yield

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After circRNA synthesis with 20 mM Mg2+, 25 mM Mg2+, 30 mM Mg2+, 35 mM Mg2+, and 40 mM Mg2+ as the cofactor, the RNA product yield was measured using a Nano-300 instrument. dsRNA content was measured using a dsRNA quantitative kit supplied by Vazyme Co., Ltd (Nanjing, China). This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect dsRNA content in the in vitro transcription system.
He W., Zhang X., Zou Y., Li J., Chang L., He Y.C., Jin Q, & Ye J. (2024). Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst. Frontiers in Bioengineering and Biotechnology, 12, 1356354.
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