Mthfd2

Manufactured by Proteintech

MTHFD2 is a product offered by Proteintech. It is a protein that is involved in one-carbon metabolism, specifically in the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide. The product is intended for research use only and its specific applications should be determined by the user.

Automatically generated - may contain errors

Lab products found in correlation

Dhcr7, by Abcam (1 mentions) Lamin a, by Santa Cruz Biotechnology (1 mentions) Phosphorylated nrf2, by Abcam (1 mentions) Shmt1, by Abcam (1 mentions) Pnpla3, by Abcam (1 mentions) Alexa fluor 568 labeled goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions) Gapdh, by Abcam (1 mentions) β actin, by Abcam (1 mentions) Asparagine, by Merck Group (1 mentions) Serine, by Merck Group (1 mentions) Formate, by Merck Group (1 mentions) Asparaginase, by Merck Group (1 mentions) Tunicamycin, by Enzo Life Sciences (1 mentions) Glycine, by Avantor (1 mentions) Rapamycin, by Merck Group (1 mentions) Pecam 1, by BD (1 mentions) Torin2, by Selleck Chemicals (1 mentions) Flag tag (flag), by BD (1 mentions) Vegf a165, by R&D Systems (1 mentions) Ml385, by Merck Group (1 mentions)

2 protocols using mthfd2

Comprehensive Antibody Panel for HCV Pathways

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Primary antibodies were for HCV proteins: core (Institute of immunology), NS5A (Antiprot), NS3 (Antiprot), NS5A (Virogen), and NS5B (Antiprot), beta-Actin (Abcam), ACYL (Cell signaling), HMGCR (Atlas antibodies), DHCR7 (Abcam), ACACA (Santa Cruz), ELOVL5 (Novusbio), GPAM (Abcam), MTTP (Abcam), PNPLA3 (Abcam), GCK (Santa Cruz), GAPDH (Abcam), G6PD (Santa Cruz), PPAT (Proteintech), MTHFD2 (Proteintech), PSAT1 (Santa Cruz), SHMT1 (Abcam), G6PC3 (Santa Cruz), DLAT (Santa Cruz), IDH3G (Santa Cruz), ASNS (Santa Cruz), ME1 (Abcam), PCK2 (Abcam), GPT2 (Santa Cruz), NQO1 (Proteintech), GCLC (Abnova), Nrf2 (Santa Cruz), phosphorylated-Nrf2 (Abcam), Maf-G (Abcam), Lamin A (Santa Cruz). HRP-labeled secondary antibodies were used against mouse IgG, rabbit IgG, and goat IgG (Amersham) dependent on the primary antibodies for immunoblot. Alexa-fluor-568-labeled goat anti-mouse secondary antibody (Invitrogen) was used for immunofluorescence.

Sugiyama K., Ebinuma H., Nakamoto N., Sakasegawa N., Murakami Y., Chu P.S., Usui S., Ishibashi Y., Wakayama Y., Taniki N., Murata H., Saito Y., Fukasawa M., Saito K., Yamagishi Y., Wakita T., Takaku H., Hibi T., Saito H, & Kanai T. (2014). Prominent Steatosis with Hypermetabolism of the Cell Line Permissive for Years of Infection with Hepatitis C Virus. PLoS ONE, 9(4), e94460.

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Metabolic Reprogramming in Cancer Cells

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The following reagents were used: NAC (Sigma-Aldrich, Cat# A7250), tunicamycin (Enzo Life Sciences, Cat# BML-CC104), glycine (VWR, Cat# 10119CU), tBHQ (Sigma-Aldrich, Cat# 19986), ML385 (Sigma-Aldrich, Cat# SML1833), Rapamycin (Sigma-Aldrich, Cat# R0395), Torin 2 (Selleckchem, Cat# S2817), serine (Sigma-Aldrich, Cat# S4500), asparagine (Sigma-Aldrich, Cat# A4159), VEGF-A 165 (R&D, Cat# 293-VE), Asparaginase (Sigma-Aldrich, Cat# A3809), l-Histidinol (Sigma-Aldrich, Cat# H6647), FFA (Sigma-Aldrich, Cat# F9905), α-glycyrrhetinic acid (Sigma-Aldrich, Cat# G10105), A438079 (Sigma-Aldrich, Cat# A9736), Oligomycin A (Sigma-Aldrich, Cat# 75351), CCCP (Sigma-Aldrich, Cat# C2759), antimycin A (Sigma-Aldrich, Cat# A8674), rotenone (Sigma-Aldrich, Cat# R8875), formate (Sigma-Aldrich, Cat# 06473), folate (Enzo Life Sciences, Cat# ALX-460-006). The following antibodies were used: pS6 (Cell Signaling, Cat# #2215, dilution 1:1000), S6 (Cell Signaling, Cat# 2317, dilution 1:1000), MTHFD2 (Proteintech, Cat# 12270-1-AP, WB dilution 1:1000, IF dilution 1:200), SHMT2 (Bethyl, Cat# A305-351A, dilution 1:2000), β-actin (Cell Signaling, Cat# 4970S, dilution 1:1000), Pecam-1 (BD Biosciences, Cat# 553370, dilution 1:200), FLAG (Sigma-Aldrich, Cat# F3165, dilution 1:1500). Primers for qRT-PCR are listed in Supplementary Table 9.

Hitzel J., Lee E., Zhang Y., Bibli S.I., Li X., Zukunft S., Pflüger B., Hu J., Schürmann C., Vasconez A.E., Oo J.A., Kratzer A., Kumar S., Rezende F., Josipovic I., Thomas D., Giral H., Schreiber Y., Geisslinger G., Fork C., Yang X., Sigala F., Romanoski C.E., Kroll J., Jo H., Landmesser U., Lusis A.J., Namgaladze D., Fleming I., Leisegang M.S., Zhu J, & Brandes R.P. (2018). Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells. Nature Communications, 9, 2292.

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