The animals were anesthetized with carbon dioxide, and blood, liver, colon, perirenal fat, mesentery fat, and epididymal fat tissues were collected. The blood sample was collected in sterile anticoagulation tube (BD Biosciences, USA). Then, 50 μL of blood was immediately taken out and stored at −80 ℃ for quantification of Bacterial DNA. The residue blood was centrifuged at 3000 r/min for 15 min at 4 ℃ to afford plasma. After weighing and taking picture, a part of the liver, colon, and epididymal fat tissues were fixed in 4% paraformaldehyde solution for histological analysis. Other tissues were stored at −80 ℃ for further experiment.
Sterile anticoagulation tube
Manufactured by BD
Sourced in United States
Sterile anticoagulation tube is a laboratory equipment used for collecting and storing blood samples. It contains an anticoagulant agent to prevent the blood from clotting during the collection and storage process.
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Lab products found in correlation
2 protocols using sterile anticoagulation tube
1
Tissue Collection for Metabolic Analysis
2
Tissue Collection for Microbiome Analysis
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The animals were anesthetized with carbon dioxide, and blood, liver, colon, perirenal fat, mesentery fat, and epididymal fat tissues were collected. The blood sample was collected in sterile anticoagulation tube (BD Biosciences, USA). Then, 50 mL of blood was immediately taken out and stored at -80 ℃ for quanti cation of Bacterial DNA. The residue blood was centrifuged at 3000 r/min for 15 min at 4 ℃ to afford plasma. After weighing and taking picture, a part of the liver, colon, and epididymal fat tissues were xed in 4% paraformaldehyde solution for histological analysis. Other tissues were stored at -80 ℃ for further experiment.
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