Genesis 10

Manufactured by Thermo Fisher Scientific

Sourced in United States, Sweden

The Genesis 10S is a UV-Vis spectrophotometer designed for general laboratory use. It features a wavelength range of 190 to 1100 nm and can perform absorbance, transmittance, and concentration measurements. The instrument provides accurate and reproducible results with a wavelength accuracy of ±0.1 nm and a photometric accuracy of ±0.002 Abs. The Genesis 10S is compatible with a variety of sample holders and cuvettes, making it a versatile tool for a wide range of applications.

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Genesis 10S UV-Vis Spectrophotometer (16 citations) GENESIS 10S UV-VIS (12 citations) Genesis 10S Bio spectrophotometer (2 citations) Genesis 10S spectrophotometer (2 citations) Genesis 10 UV spectrophotometer (2 citations) Genesis 10 spectrophotometer (2 citations)

9 protocols using genesis 10

Optimizing GUL and DOTA-Gd Conjugation

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In order to facilitate optimisation and analysis of the conjugation of GUL, in a separate synthesis, as described above, para-amino-benzoic acid (PABA, Sigma Aldrich, Darmstadt, Germany) was included in the GUL synthesis i.e. PABA was substituted to the lysine residue of GUL (Peptides & Elephants, Potsdam, Germany) and conjugation to dextran was done through the aromatic amine of PABA. Hence a PABA standard curve was prepared at A280 using a spectrophotometer (Genesis 10S, ThermoFisher Scientific, Göteborg, Sweden) and was used for determination of GUL conjugate substitution.
PDC, conjugation of radio nuclide metal chelator: To demonstrate the versatility of the PDC conjugate, Gd.(2-4aminobenzyl)-1,4,7,10tetraaazacyclodocecane-1,4,7,10-tetraacetate (DOTA, from Macrocyclics inc, Texas, USA) was included in the conjugation synthesis described above i.e. after the GUL coupling, 40 mg DOTA was added, together with 12 mg lysine. Hence, a DOTA standard curve was prepared at A280 using a spectrophotometer (Genesis 10S, ThermoFisher Scientific, Göteborg, Sweden) and was used for determination of DOTA-Gd conjugate substitution.

Holmberg A.R., Marquez M., Lennartsson L., Meurling L, & Nilsson S. (2018). Synthesis and Binding of a Novel PSMA-specific Conjugate. Anticancer research, 38(3).

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Quantifying Total Polyphenols in Plant Extracts

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The crude extracts obtained in each trial were assayed for quantifying the total polyphenols content according to the method referred to by Nossa González et al. [56 ] as follows: 125 μL of sample, 500 μL of distilled water and 125 μL of Folin–Ciocalteu reagent were added in a test tube. The solution was left for 6 min at room temperature to react. Then, 1.25 mL of Na₂CO₃ solution (7%) and 1 mL of distilled water were added. The mixture was left to react at room temperature (25 °C) for 90 min. The reaction was monitored at 760 nm in a UV-vis spectrophotometer (Genesis 10, Thermo Scientific, Waltham, MA, USA). The gallic acid standard curve (0–100 μg/mL) was used as a reference [57 (link)]. The results were expressed in GAE/100 g of dried plant material.

Gutiérrez-Sánchez M.D., Aguilar-Zárate P., Michel-Michel M.R., Ascacio-Valdés J.A, & Reyes-Munguía A. (2022). The Ultrasound-Assisted Extraction of Polyphenols from Mexican Firecracker (Hamelia patens Jacq.): Evaluation of Bioactivities and Identification of Phytochemicals by HPLC-ESI-MS. Molecules, 27(24), 8845.

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Particle Concentration Determination via Turbidity

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In the case of low‐concentration particle suspensions, predominantly in the filtrate, the concentration determination via dry mass measurement reaches its detection limit. Therefore, to determine the concentration of particle suspensions, turbidity measurements were performed using an UV/Vis spectrometer (Genesis 10, Thermo Scientific Inc.) at a wavelength of λ = 600 nm. The measurements took place in 10 × 4 × 45 mm half‐micro cuvettes made of polystyrene (Sarstedt AG & Co. KG). If necessary, samples were diluted with deionized water or respective buffer. The filtrate produced by the HGMS was measured undiluted.

Wommer L., Meiers P., Kockler I., Ulber R, & Kampeis P. (2021). Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles. Engineering in Life Sciences, 21(10), 573-588.

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Turbidity Measurements of Particle Suspensions

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An UV/Vis spectrometer (Genesis 10, Thermo Scientific) at a wavelength of 600 nm was used for turbidity measurements of particle suspensions. Measurements were made in 10 × 4 × 45 mm half‐micro polystyrene cuvettes (Sarstedt AG & Co. KG). Turbidity measurements were performed using a 1:40 dilution with deionized water or the buffer system used. The (magnetic) filtrate resulting from the HGMS was measured undiluted.

Wommer L., Soerjawinata W., Ulber R, & Kampeis P. (2021). Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification. Engineering in Life Sciences, 21(10), 558-572.

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Ferrozine Assay for Fe(III) Reduction

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To measure the reduction of Fe(III) into Fe(II), the ferrozine assay was performed following a modified protocol from Bell et al.^{41 (link)}. First, 100 µL of 5 M HCl were added immediately after sampling to the 900 µL of collected samples and stored at 4 °C. The samples were centrifuged for 1 min at 6700 × g, then 100 µL of the supernatant were taken and 900 µL of ferrozine solution (0.1% ferrozine in a 100 mM HEPES solution at pH 7) were added. Fe(II) concentration was determined by measuring the absorbance at 562 nm with a UV-Vis spectrophotometer (Thermo Scientific Genesis 10 s). A calibration curve was obtained using serial dilutions of 1 mM ferrous ammonium sulphate solution in acidic MilliQ water (pH 2).

Kooli W.M., Comensoli L., Maillard J., Albini M., Gelb A., Junier P, & Joseph E. (2018). Bacterial iron reduction and biogenic mineral formation for the stabilisation of corroded iron objects. Scientific Reports, 8, 764.

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Laccase Activity Measurement Protocol

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Laccase activity measurements were conducted in accordance to the method reported by Niku-Paavola et al. [49 (link)]. pH 4.0 phosphate buffer solution was prepared from KH₂PO₄ and K₂HPO₄. Kinetic spectrophotometric measurements were performed in a Genesis 10S spectrophotometer (Thermo Fisher Scientific, Walthman, MA, USA) for 2 min at 436 nm. One activity unit was defined as the amount of laccase needed to oxidize 1 µmol of ABTS per min. Laccase activity was expressed in terms of units per liter (U L⁻¹) and was set at 1100 U L⁻¹.

Lopez-Barbosa N., Florez S.L., Cruz J.C., Ornelas-Soto N, & Osma J.F. (2020). Congo Red Decolorization Using Textile Filters and Laccase-Based Nanocomposites in Continuous Flow Bioreactors. Nanomaterials, 10(6), 1227.

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Quantifying Fe(III) Reduction using Ferrozine

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To measure the reduction of Fe(III) into Fe(II), the ferrozine assay was performed following a protocol modified from that of Bell et al.(42 (link)). First, 100 μl of 5 M HCl was added to 900 μl of collected samples and stored at 4°C. The samples were centrifuged for 1 min at 6,700 × g, and then 100 μl of the supernatant was taken and 900 μl of ferrozine solution (0.1% ferrozine in a 100 mM HEPES solution at pH 7) was added. The Fe(II) concentration was determined by measuring the absorbance at 562 nm with a UV-visible spectrophotometer (Thermo Fisher Scientific Genesis 10s). A calibration curve was obtained using serial dilutions of 1 mM ferrous ammonium sulfate solution in acidic MilliQ water (pH 2).

Kooli W.M., Junier T., Shakya M., Monachon M., Davenport K.W., Vaideeswaran K., Vernudachi A., Marozau I., Monrouzeau T., Gleasner C.D., McMurry K., Lienhard R., Rufener L., Perret J.L., Sereda O., Chain P.S., Joseph E, & Junier P. (2019). Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates. Applied and Environmental Microbiology, 85(3), e02042-18.

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Physico-chemical Analysis of Wine Samples

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Physico-chemical parameters of the wine samples such as the content of total sugar, total acidity, and volatile acid were measured according to the analytical methods of wine and fruit wine (National Standard of the People’s Republic of China) [38 ]. The total sugar was measured by Fehling regent titration method whiles the total acidity and volatile acid were measured by acid–alkali titration method, and phenolphthalein was used as the indicator. The transmittance was measured by a Genesis 10S ultraviolet-visible spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) at 680 nm. The color intensity and total phenol content were measured according to the Compendium of International Methods of Wine and Must Analysis methods by measuring absorbance at 420 nm and 750 nm (10 mm cell) respectively using the UV-VIS spectrophotometer [39 ]. The content of protein was measured by Bradford assay [40 (link)].

Ma T.Z., Gong P.F., Lu R.R., Zhang B., Morata A, & Han S.Y. (2020). Effect of Different Clarification Treatments on the Volatile Composition and Aromatic Attributes of ‘Italian Riesling’ Icewine. Molecules, 25(11), 2657.

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Ferrochelatase Activity Assay Protocol

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1 mL of protein extract was incubated at 37°C in a Thunberg tube in a 4.2 mL reaction volume containing 200 µmol porphyrin substrate, 400 µmol FeSO4, 40 µmol GSH and 200 µmol phosphate buffer (pH 7.8). After incubation, the reaction was stopped by adding 1 mL of pyridine, 0.5 mL of 1 N NaOH and 1 mL of water. The reaction mixture was divided into two equal portions. In the first part, 2 mg of solid Na2S204 and in the second part, 0.05 mL of 3 mM K3Fe(CN)6 were added and analyzed in UV-VIS spectrophotometer (Thermo Genesis 10S) (Porra and Jones, 1963) . One unit of ferrochelatase activity is the amount of enzyme that catalyzes the formation of 1 nmol metalloprotoporphyrin at 1 h at 37°C.

Konar V., İdil Ö., Çelikoğlu E., & Çelikoğlu U. (2020). Ferro-chelatase enzyme activity of blue green algae from Yeşilırmak. International Journal of Science Letters, 2(2), 72-78.

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