All human ovarian carcinoma cell lines were obtained from American Type Culture Collection (ATCC). CaOV3, OVCAR3, SKOV3 were grown in Dulbecco’s modified essential medium (DMEM, Cellgro). OV90, OVCA429, UCI101 were cultured in RPMI-1640 medium (Cellgro). ES-2 were cultured in McCoy 5a medium, TOV112D and TOV21G in 1:1 mixture of MCDB 105 medium and Medium 199 (Sigma-Aldrich), and UWB1.289 in 1:1 mixture of RPMI-1640 medium and mammary epithelial growth medium (MEGM, Lonza). All media were supplemented with 10% fetal bovine serum (FBS) and penicillin-streptomycin (Cellgro). Mesothelial cells, LP3 and LP9, were purchased from the Corriell Institute for Medical Research and grown according to protocol. Normal ovarian surface epithelium (NOSE) cell lines were derived from ovarian surface epithelium (OSE) brushings and cultured in 1:1 mixture of MCDB 105 medium and Medium 199 (Sigma-Aldrich) with gentamicin (25 μg/mL) and 15% heat-inactivated serum. TIOSE4 and TIOSE 6 cell lines were then obtained by transfecting NOSE cells with hTERT, and cultured in RPMI-1640 supplemented with 10% FBS and penicillin-streptomycin. All cell lines were tested and free of mycoplasma contamination (MycoAlert Mycoplasma Detection Kit, Lonza, LT07-418).
Tov112d
Manufactured by Merck Group
The TOV112D is a laboratory equipment product manufactured by Merck Group. It is a specialized device designed for specific applications in scientific research and testing environments. The core function of the TOV112D is to perform precise measurements and analyses, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Mcdb105 medium, by Merck Group (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Medium 199, by Merck Group (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Rpmi 1640 medium, by Lonza (1 mentions)
Calf serum, by Thermo Fisher Scientific (1 mentions)
Tov 21g, by Merck Group (1 mentions)
Mcdb 105, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Medium 199, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Merck Group (1 mentions)
2 protocols using tov112d
1
Ovarian Cancer Cell Line Characterization
2
Ovarian Cancer Cell Line Cultivation
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The human ovarian papillary serous cystoadenocarcinoma cell line, OC314, was obtained from the ICLC Animal Cell Lines Database (Servizio Biotecnologie IST, Centro di Biotecnologie, Avanzate L.go R. Benzi, Genova, Italy). Cells were propagated in RPMI 1640 medium (Life Technologies, Rockville, MD, USA) supplemented with 5% fetal bovine serum (FBS; Life Technologies) and 2 mM L-glutamine (Sigma-Aldrich, St. Louis, MO, USA). Other human cell lines including TOV-112D (derived from an ovarian endometrioid carcinoma), TOV-21G (derived from an ovarian clear cell carcinoma), H184B5H5/M10 (human mammary epithelial cells), T/G HA-VSMC (human normal aorta smooth muscle cells), and HFL1 (lung fibroblasts) were obtained from the Bioresources Collection and Research Center (BCRC, Hsinchu, Taiwan). TOV-112D and TOV-21G cells were propagated in a 1:1 mixed medium of MCDB 105 (Sigma-Aldrich) and Medium 199 (Life Technologies) supplemented with 15% FBS. H184B5H5/M10 cells were propagated in GIBCO 11900 medium (Life Technologies) supplemented with 10% calf serum (Life Technologies). HFL1 and T/G HA-VSMC cell lines were propagated in Ham’s F12K medium (HyClone, Logan, UT, USA) supplemented with 10% FBS.
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