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Water 600 binary pump

Manufactured by Waters Corporation
Sourced in United States

The Waters 600 binary pump is a high-performance liquid chromatography (HPLC) pump designed to deliver precise and reproducible flow rates of up to 10 mL/min. It features two independent, high-precision syringe-type pump mechanisms that allow for the delivery of binary solvent gradients. The pump is capable of operating at pressures up to 6,000 psi, making it suitable for a wide range of HPLC applications.

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2 protocols using water 600 binary pump

1

HPLC Analysis of Organic Compounds

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A Waters High performance liquid chromatography (HPLC) system equipped with a Water 600 binary pump, Waters 2489 UV/detector, and Waters 717 plus autosampler (Waters Technologies Corporation, 34 Maple St., Milford, MA 0157) and a Phenomenex Luna 5um C18 (2) 250 × 4.6 mm column (Torrance, CA, USA) were used at a detection wavelength of 273 nm. The mobile phase consisted of Methanol and water at a ratio of 70:30 (v/v). The mobile phase flow rate was maintained at 1.0mL/min. and an injection volume of was 20 μL was used (O’connell and Zurzola, 1984 (link)). HPLC data was analyzed using Empower V. software (Milford, MA, USA).
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2

HPLC Analysis of Ketoprofen Quantification

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All samples were analyzed using a Waters HPLC equiped with Empower software to analyze the data. HPLC consisted of a Water 600 binary pump, Waters 2489 UV/detector, and Waters 717 plus autosampler (Waters Technologies Corporation, 34 Maple St., Milford, MA 0157). The column used was phenomenex luna C18 (5µ, 250 mm × 4.6 mm). The mobile phase constituted of acetonitrile/20 mMol phosphate buffer, 55:45 (%v/v) at pH 430 (link), 31 (link) at a flow rate of 1 ml/min and injection volume of 20 µl. The UV detetctor wavelength for KTP detection was set at 256 nm.
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