Phospho stat3 tyr705

Manufactured by Abcam

Sourced in United Kingdom

Phospho-STAT3 (Tyr705) is a lab equipment product that detects the phosphorylation of STAT3 protein at tyrosine residue 705. This modification is important for STAT3 activation and signaling. The product can be used to measure the activation status of STAT3 in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Ecl plus, by Thermo Fisher Scientific (3 mentions) Stat3, by Abcam (2 mentions) Il 1β, by Sino Biological (1 mentions) Phospho jak2, by Proteintech (1 mentions) Tnf α, by Sino Biological (1 mentions) Vegfa, by Novus Biologicals (1 mentions) Total stat3, by Abcam (1 mentions) Interleukin 6 (il 6), by Bioworld Technology (1 mentions) Hif 1a, by Novus Biologicals (1 mentions) Hif2a, by Cell Signaling Technology (1 mentions) Gp130, by Abcam (1 mentions) Nc membrane, by Merck Group (1 mentions) Ecl western blotting reagent, by Thermo Fisher Scientific (1 mentions) Bcl 2, by Abcam (1 mentions) Streptavidin agarose, by Thermo Fisher Scientific (1 mentions) Anti ha affinity gel, by Merck Group (1 mentions) Universal nuclease, by Thermo Fisher Scientific (1 mentions) Phosphatase inhibitor cocktail, by Merck Group (1 mentions) Na k atpase, by Santa Cruz Biotechnology (1 mentions) Bradford assay, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-Phospho-STAT3 (Tyr705) Phospho-STAT3 STAT3

5 protocols using phospho stat3 tyr705

Signaling Pathways in Liver and AML12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The rat livers and AML12 cells were lysed by RIPA buffer, separated by 10% polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride (PVDF) membrane. After blocking in 5% Bovine Serum Albumin (BSA), the membrane was incubated with the phospho-JAK2 (Tyr931) (1:1,000, affinity), total JAK2 (1:1,000, proteintech), phospho-STAT3 (Tyr705) (1:1,500, abcam), total STAT3 (1:2000, abcam), IL-6 (1:500, bioworld), IL-18 (1:2000, bioworld), IL-1β (1:500, sino biological), TNFα (1:1,000, sino biological), HIF-1a (1:1,000, Novusbio), HIF-2a (1:1,000, Cell Signaling Technology), VEGFa (1:1,000, Novusbio) primary antibody seperately overnight at 4°C, and then was incubated with HRP conjugated secondary antibody for 1 h at room temperature. Western blots were displayed by advanced enhanced chemiluminescence kit and then semi-quantified by Clinx (Shanghai Qinxiang Scientific Instrument Co., Ltd).

Zhang M., Wu W., Huang C., Cai T., Zhao N., Liu S, & Yang S. (2022). Shuxie-1 Decoction Alleviated CUMS -Induced Liver Injury via IL-6/JAK2/STAT3 Signaling. Frontiers in Pharmacology, 13, 848355.

+ Open protocol

+ Expand

Analyzing JAK-STAT Signaling in Gastric Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole cell lysates were prepared from BGC823 or SGC-7901 cells treated as previous designed and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis at 100 V for 1 hour. Next, the separated proteins were transferred to NC membranes (Millipore, Billerica, MA). The samples were blocked with 5% bovine serum albumin (BSA) in TBS containing 0.1% Tween-20 for 1 hour at room temperature and incubated overnight at 4°C with one of the following antibodies: JAK (1:200, Abcam, Cambridge, UK), phospho JAK (Ser473) (1:200, Abcam), STAT3 (1:300, Abcam), phospho STAT3 (Tyr705) (1:300, Abcam), BCL-2 (1:200, Abcam), GP130 (1:300, Abcam) and actin (1:500, Abcam). The samples were washed with TBST and incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature. Proteins were visualized by ECL western blotting reagent (Thermo Fisher).

Ma J., Song X., Xu X, & Mou Y. (2018). Cancer-Associated Fibroblasts Promote the Chemo-resistance in Gastric Cancer through Secreting IL-11 Targeting JAK/STAT3/Bcl2 Pathway. Cancer Research and Treatment : Official Journal of Korean Cancer Association, 51(1), 194-210.

+ Open protocol

+ Expand

Immunoprecipitation and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents and antibodies were purchased from commercial sources: inhibitor cocktail (Trichostatin A (TSA, T8552, Sigma), protease inhibitor cocktail (P8340, Sigma), phosphatase inhibitor cocktail (P0044, Sigma)), fedratinib (S2736, Selleckchem), universal nuclease (88700, Thermo Fisher), Bradford assay (23200, Thermo Fisher), dithiothreitol (DTT; DTT100, Goldbio), enzyme-linked chemiluminescence (ECL) plus (32132, Thermo Fisher), SYBR Green PCR Master Mix (4472908, Applied Biosystems), streptavidin agarose (20359, Thermo Fisher), Protein A/G PLUS-Agarose (sc-2003, Santa Cruz Biotechnology), anti-Flag agarose gel (A2220, Sigma) and anti-HA affinity gel (E6779, Sigma). Antibodies were as follows: STAT3 (9139, CST), phospho-STAT3 (Tyr705) (ab76315, Abcam), β-actin (C4) HRP (SC-47778, Santa Cruz), Na/K-ATPase (SC-21712, Santa Cruz), histone H3 (4499S, CST), Flag HRP (A8592, Millipore), HA-probe (Y-11) (SC805, Santa Cruz), HA-probe (F-7) (SC7392, Santa Cruz), DHHC7 (ab138210, Abcam), DHHC7 (R12–3691, Assay Biotechnology), Alexa Fluor 350 goat anti-rabbit IgG (A-11046, Invitrogen), Alexa Fluor 594 goat anti-mouse IgG (8890S, CST), mouse CD4 PerCP-Cy5.5 (560767, BD Pharmingen), mouse IL-17A PE (560767, BD Pharmingen), anti-mouse IgG HRP (7076S, CST) and anti-rabbit IgG HRP (7074S, CST).

Zhang M., Zhou L., Xu Y., Yang M., Xu Y., Komaniecki G.P., Kosciuk T., Chen X., Lu X., Zou X., Linder M.E, & Lin H. (2020). A STAT3 palmitoylation cycle promotes TH17 differentiation and colitis. Nature, 586(7829), 434-439.

+ Open protocol

+ Expand

Multiplex Immunoassay for Protein Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunohistochemical, immunocytological and/or immunoblotting assays, the following antibodies were used against: αβ tubulin, LC3B, phospho-p44/42 MAPK (ERK1/2) (Thr 202, Tyr 204), phospho-RICTOR (Thr 1135), phospho-S6RP (Ser 235) and purchased from Cell Signaling Technology (Ozyme, St-Quentin-en-Yvelines, France). GLUT1, HIF-1α, HIF-2α, PARP, cleaved PARP, phospho-STAT3 (Tyr705) were purchased from Abcam (Paris, France), and phospho-H2AX (Ser 139) from Merck Millipore (Darmstadt, Germany). The histone status was assessed by immunohistochemical staining with an antibody detecting the loss of nuclear expression of the trimethylated lysine 27 (H3K27me3) in histone H3 (Diagenode, Belgium).

Nguyen A., Moussallieh F.M., Mackay A., Cicek A.E., Coca A., Chenard M.P., Weingertner N., Lhermitte B., Letouzé E., Guérin E., Pencreach E., Jannier S., Guenot D., Namer I.J., Jones C, & Entz-Werlé N. (2017). Characterization of the transcriptional and metabolic responses of pediatric high grade gliomas to mTOR-HIF-1α axis inhibition. Oncotarget, 8(42), 71597-71617.

+ Open protocol

+ Expand

Western Blot Analysis of NF-κB Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cells were harvested, washed, and lysed with RIPA lysis buffer containing a protease inhibitor cocktail (BBI Life Sciences, Shanghai, China) together with NaF and Na₄P₂O₇, and the protein concentration was measured using a BCA Protein Assay Kit (Pierce, Waltham, MA, USA). Subsequently, the samples were loaded onto SDS-PAGE gels and separated through electrophoresis. The proteins were transferred onto PVDF membranes and incubated with the corresponding primary Abs and HRP-conjugated secondary Abs. Chemiluminescent detection was performed using Alliance 4.7 (UVITEC Cambridge, Cambridge, UK) with Luminata Forte Western HRP Substrate (Millipore, Billerica, MA, USA).
The following antibodies were used: Phospho-IκBα (Ser32) (CST#2859, Cell Signaling Technology, Danvers, MA, USA), IκBα (CST#4812), Phospho-NF-κB p65 (Ser536) (CST#3033), NF-κB p65 (CST#3034), NIK (CST#4994), NF-κB2 p100/p52 (CST#4882), RelB (CST#4922), Phospho-Stat3 (Tyr705) (CST#9131), Phospho-Stat3 (Ser727) (CST#9134), SHP-1 (CST#3759), SHP-2 (CST#3397), Stat-3 (ab68153, Abcam, Cambridge, UK), β-Actin (ACTB) (BM0627, Boster, Wuhan, China), HRP Anti-rabbit IgG (CST#7074), and HRP anti-mouse IgG (BioLegend, 405306).

Li R., Fang F., Jiang M., Wang C., Ma J., Kang W., Zhang Q., Miao Y., Wang D., Guo Y., Zhang L., Guo Y., Zhao H., Yang D., Tian Z, & Xiao W. (2017). STAT3 and NF-κB are Simultaneously Suppressed in Dendritic Cells in Lung Cancer. Scientific Reports, 7, 45395.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!