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Ab155668

Manufactured by Abcam
Sourced in United Kingdom

The Ab155668 is a laboratory equipment product from Abcam. It is designed for general laboratory use.

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2 protocols using ab155668

1

Antibody Sources for Cell Signaling

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mouse monoclonal antibodies against GST (B-14), Rod (43-K), and CENP-B (F-4; Fig. 1 E and Fig. S4 G) and rabbit polyclonal antibodies against CENP-B (H-65), Cyclin B1 (H-433), His (H-15), and GFP (sc-8334) were obtained from Santa Cruz Biotechnology. Antibodies against Mad1 (ab5783, mouse monoclonal), BubR1 (ab4637, rabbit polyclonal, used for Western blotting in Fig. S3 G), and Chmp4c (ab155668, rabbit polyclonal) were obtained from Abcam. mouse monoclonal antibodies against CENP-A (3–19; GTX13939) and Hec1 (9G3.23; GTX70268) were obtained from Genetex, and mouse monoclonal antibodies against α-tubulin (DM1A) and actin (AC-40) were obtained from Sigma-Aldrich. Anti-pS331 is a rabbit polyclonal antiserum raised against phosphorylated S331 of human Aurora-B (Petsalaki et al., 2011 (link)). A sheep polyclonal antibody against human BubR1 (used for immunofluorescence) was from S. Taylor (University of Manchester, Manchester, England, UK), and rabbit polyclonal antibodies against human ZW10 and Zwilch were from A. Musacchio (Max Planck Institute of Molecular Physiology, Dortmund, Germany). Anti–pChmp4c rabbit polyclonal antiserum against phosphorylated S210, S214, and S215 of human Chmp4c was a gift from P.P. D’Avino (University of Cambridge, Cambridge, England, UK; Capalbo et al., 2012 (link)).
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2

Antibody Sources and Dilutions for Research

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Antibodies were purchased and used as follows: commercial mouse monoclonal antibodies against ALIX (sc-53540; Santa Cruz Biotechnology, Dallas, TX; 1:500), Flag (M2, F3165; Sigma, St. Louis, MO; 1:1000), α-tubulin (DM1A, T9026; Sigma; 1:1000), lamin A/C (sc-7292; Santa Cruz Biotechnology; 1:2000), Emerin (4G5, MS-1751-S; Lab Vision Corporation, Fremont, CA; 1:1000), gB (P1105; Virusys Corporation, Taneytown, MD; 1:2000), Strep-tag (4F1, M211-3; MBL, Nagoya, Japan; 1:1000), and DFz2 (12A7; DSHB, Iowa City, IA; 1:6); commercial rabbit polyclonal antibody against GFP (598; MBL; 1:1000), CHMP4B (ab105767; Abcam, Cambridge, UK; 1:1000), CHMP4C (ab155668; Abcam; 1:500), lamin B1 (ab16048-100; Abcam; 1:1000) and calnexin (c4731; Sigma; 1:2000); and commercial mouse polyclonal antibodies against CHMP4A (ab67058; Abcam; 1:500). Mouse polyclonal antibodies against UL31(1:500) and rabbit polyclonal antibodies against UL34 (1:2000) were reported previously61 (link).
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