The cell viability of the cultured BSMCs was measured by MTT kit (Amresco, USA) according to the manufacturer’s instructions.
Mtt kit
Manufactured by Avantor
Sourced in United States
The MTT kit is a colorimetric assay used to measure cell metabolic activity. It utilizes the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound, which is reduced by viable cells to form a purple formazan product. The intensity of the color produced is proportional to the number of metabolically active cells in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Thermo Fisher Scientific (2 mentions)
Imark plate reader, by Bio-Rad (1 mentions)
Epigallocatechin gallate (egcg), by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Hyclone fetal bovine serum, by GE Healthcare (1 mentions)
Flow cytometry, by Beckman Coulter (1 mentions)
Collagenase type 2, by Worthington (1 mentions)
Jem 2000ex transmission electron microscope, by JEOL (1 mentions)
4 protocols using mtt kit
1
MTT Assay for BSMC Viability
2
MTT Cell Viability Assay
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Cell viability was evaluated by use of the MTT kit (Amresco, OH) according to the manufacture’s protocol. Briefly, 200 μl (1×104/ml) cells were seeded into 96-well plates and cultured for 24, 48, or 72 h. After the addition of 20 μl (5 mg/ml) MTT into each well, cells were cultured for another 4 h at 37°C in an incubator. The culture medium was aspirated and the wells were washed with PBS and dried. Finally, 150 μl DMSO was added into each well. The dye was dissolved thoroughly by shaking. Absorbance at 570 nm was read by a BioRad iMark plate reader.
3
Bioactive Nanocomposite Synthesis and Evaluation
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All experimental materials were used as received with no purification. MTT kit was purchased from Amresco Inc. (Solon, OH, USA). Casein phosphopeptide–amorphous calcium phosphate (CPP–ACP) contained commercial paste Tooth Mousse was purchased from GC Corp. (Tokyo, Japan). Brain heart infusion (BHI) and agar powder were obtained from Difco, Becton-Dickinson (Sparks, MD, USA). EGCG, MSN, and dimethyl sulfoxide (DMSO) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Glutaraldehyde, sucrose, citric acid monohydrate, ethylenediamine tetraacetic acid (EDTA), absolute ethanol, and phosphate-buffered saline (PBS) were obtained from Aladdin Bio-Chem Technology (Shanghai, China). Ammonium phosphate [(NH4)2HPO4] and calcium nitrate tetrahydrate [Ca(NO3)2·4H2O] were supplied by Kermel Chemical Reagent (Tianjin, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan).
4
Chondroprotective Effects of Proanthocyanidins
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Reagents and equipment. DMEM/F12 medium, recombinant mouse IL-1β was obtained from Bioengineering Co., Ltd. (Songjiang, Shanghai, China); HyClone™ fetal bovine serum was obtained from GE Healthcare (Logan, UT, US); type II collagenase was obtained from Worthington Biochemical Corp. (Lakewood, NJ, USA); oligomeric proanthocyanidins were purchased from Weikeqi Biological Ltd. (Chengdu, Sichuan, China); trypsin from Gibco (Grand Island, NY, USA); MTT kit from Amresco (Solon, OH, USA); reactive oxygen species detection kit (DCFH-DA), mitochondrial membrane potential assay kit (5' ,6,6'-tetrachloro-1,1' ,3,3'-tetraethylbenzimidazolylcabocyanine iodide, JC-1) obtained from Beyotime Institute of Biotechnology (Songjiang, Shanghai, China); flow cytometry apoptotic detection kit from BD Biosciences (Lake Franklin, NJ, USA); microplate reader from Thermo Fisher Scientific Inc. (Waltham, MA, USA); flow cytometry from Beckman Coulter, Inc. (Brea, CA, USA); JEM-2000EX transmission electron microscope from JEOL Ltd. (Akishima, Tokyo, Japan).
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