Harris hematoxylin

Manufactured by Muto Pure Chemicals

Sourced in Japan

Harris' Hematoxylin is a laboratory stain used in histological and cytological applications. It is a nuclear stain that selectively colors nuclei blue-purple. The stain is used to aid in the identification and visualization of cellular structures during microscopic examination.

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Lab products found in correlation

Ys100, by Nikon (2 mentions) Eosin y solution, by Merck Group (2 mentions) Anti alpha smooth muscle actin antibody, by Abcam (1 mentions) Anti s100a4 antibody, by Abcam (1 mentions) Abc kit, by Vector Laboratories (1 mentions)

3 protocols using harris hematoxylin

Ovarian Histological Analysis Protocol

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The ovaries were fixed in 4% paraformaldehyde, and then rinsed in ethanol series.
After embedding in a paraffin block, the tissue blocks were cut into 10
µm sections. The tissue blocks were deparaffinized and rehydrated by
sequentially treating xylene and ethanol, and washed with PBS. Tissue slides
were stained with Harris’ Hematoxylin (Muto Pure Chemicals, Tokyo, Japan)
and eosin Y solution (Sigma-Aldrich, St. Louis, MO, USA). All stained ovarian
sections were observed under an optical microscope (YS100, Nikon, Tokyo,
Japan).

Whang J., Ahn C., Kim S., Seok E., Yang Y., Han G., Jo H, & Yang H. (2021). Effects of Repeated Ovarian Stimulation on Ovarian Function and Aging in Mice. Development & Reproduction, 25(4), 213-223.

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Ovary and Uterus Histological Analysis

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The ovary and uterus were fixed in 4% paraformaldehyde, and then rinsed in
ethanol series. After embedding in a paraffin block, the tissue blocks were cut
into 10 μm sections. The tissue blocks were deparaffinized and rehydrated
by sequentially treating xylene, and ethanol and then washed with PBS. Tissue
slides were stained with Harris’ Hematoxylin (Muto Pure Chemicals, Tokyo,
Japan) and Eosin Y solution (Sigma-Aldrich). All stained ovarian and uterine
sections were observed under an optical microscope (YS100, Nikon, Tokyo,
Japan).

Seo J., Lee J., Kim S., Lee M, & Yang H. (2022). Lipid Polysaccharides have a Detrimental Effect on the Function of the Ovaries and Uterus in Mice through Increased Pro-Inflammatory Cytokines. Development & Reproduction, 26(4), 135-144.

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Immunohistochemical Analysis of IPF Lung Tissue

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Lung tissue sections from IPF patients were fixed in 4% buffered paraformaldehyde and embedded in paraffin. The tissue was cut into 4mm-thick slices, and the sections were deparaffinized and rehydrated in increasing concentrations of ethanol. The sections were treated with 1.4% H 2 O 2 -methanol for 30 min to block endogenous peroxidase, and non-specific binding was next blocked by incubation in 1.5% normal house serum. The sections were incubated with a monoclonal anti-alpha smooth muscle actin antibody (1:50, Abcam, Cambridge, MA) and polyclonal anti-S100A4 antibody (1:50, Abcam), followed by the ABC Kit (Vector Laboratories, Burlingame, CA). Color was developed using a Liquid DAB Substrate Kit (Golden Bridge International, Inc., Mukilteo, WA) and counterstained with Harris' hematoxylin (Muto Pure Chemicals Co., Ltd., Tokyo, Japan).

Lee J.U., Chang H.S., Shim E.Y., Park J.S., Koh E.S., Shin H.K., Park J.S, & Park C.S. (2020). The S100 calcium-binding protein A4 level is elevated in the lungs of patients with idiopathic pulmonary fibrosis. Respiratory medicine, 171.

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