Nicotinamide adenine dinucleotide phosphate nadph

Manufactured by Maclin Biochemical Technology

Sourced in China

Nicotinamide adenine dinucleotide phosphate (NADPH) is a coenzyme involved in various biological processes. It plays a crucial role in the reduction of oxidized glutathione and assists in the synthesis of fatty acids and nucleic acids. NADPH is an essential cofactor for numerous enzymatic reactions.

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Lab products found in correlation

Methanol, by Thermo Fisher Scientific (6 mentions) Formic acid, by Thermo Fisher Scientific (6 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (3 mentions) Mgcl2, by Maclin Biochemical Technology (2 mentions) Mangiferin, by Chengdu Must Bio-Technology (1 mentions) 6 well plate, by Corning (1 mentions) Oasis hlb c18 low solid phase extraction cartridges, by Waters Corporation (1 mentions) Six well plate, by Corning (1 mentions) Pterostilbene, by Chengdu Must Bio-Technology (1 mentions)

3 protocols using nicotinamide adenine dinucleotide phosphate nadph

Mangiferin Metabolic Profiling Protocol

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Mangiferin
(purity ≥98%) was purchased from Chengdu Must Biotechnology
Co., Ltd. (Sichuan, China). HPLC-grade acetonitrile, methanol, and
formic acid were purchased from Thermo Fisher Scientific (Fair Lawn,
NJ, USA). Deionized water used throughout the experiment was purchased
from Watsons (Guangzhou, China). Rat liver microsomes were obtained
from NEWGAINBIO Co., Ltd. (Wuxi, China) and Grace Pure SPE C₁₈-Low SPE cartridges (200 mg/3 mL, 59 μm, 70 Å) were purchased
from Grace Davison Discovery Science (Deerfield, IL, USA). Nicotinamide
adenine dinucleotide phosphate (NADPH) and magnesium chloride (MgCl₂) were provided by Shanghai Macklin Biochemical Co., Ltd.
(Shanghai, China). The 6-well plates were obtained from Corning Incorporated-Life
Science (Jiangsu, China).

Zhou H., Song S., Lan X., Li Y., Yuan X., Yang J., Li M., Cao T, & Zhang J. (2023). Comprehensive Profiling of Mangiferin Metabolites In Vivo and In Vitro Based on the “Drug Metabolite Clusters” Analytical Strategy. ACS Omega, 8(11), 9934-9946.

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Naringenin Metabolism in Rat Liver Microsomes

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Naringenin standard was purchased from Chengdu Must-Technology Co., Ltd. (Chengdu, China). Its purity was acceptable (≥98%) according to HPLC–UV analysis, and its structure was fully elucidated by comparing the spectral data (ESI-MS and ¹H, ¹³C-NMR spectroscopy) with the literature. HPLC-grade acetonitrile, methanol, and formic acid (FA) were purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). The deionized water used throughout the experiment was purchased from Watsons (Guangzhou, China). Oasis^® HLB C₁₈-low solid-phase extraction cartridges (500 mg·6 mL⁻¹, 60 µm, 149 Å) were purchased from Waters Corporation (Milford, MA, USA). Rat liver microsomes were obtained from NEWGAINBIO Co., Ltd. (Wuxi, China). Nicotinamide adenine dinucleotide phosphate (NADPH) and MgCl₂ were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Six-well plates were obtained from Corning Incorporated-Life Science (Hangzhou, China).

Cui Y.F., Zhang W.W., Li Y.N., Xu J., Lan X.M., Song S.Y., Lin Y.Q., Dai L, & Zhang J.Y. (2022). The Analytical Strategy of “Ion Induction and Deduction Based on Net-Hubs” for the Comprehensive Characterization of Naringenin Metabolites In Vivo and In Vitro Using a UHPLC-Q-Exactive Orbitrap Mass Spectrometer. Molecules, 27(21), 7282.

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Pterostilbene Metabolism in Rat Liver

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Pterostilbene
(purity ≥99.99%, MUST-21052207) was provided by Chengdu Must
Biotechnology Co. Ltd. (Sichuan, China); HPLC-grade acetonitrile,
methanol, and formic acid were purchased from Thermo Fisher Scientific
(Fair Lawn, NJ, USA). The deionized water used throughout the experiment
was purchased from Watsons (Guangzhou, China). Rat liver microsomes
were obtained from Xin Run Biotechnology Co., Ltd. (Wuxi, China);
Oasis HLB C18-Low solid-phase extraction (SPE) cartridges (500 mg/6
mL, 60 μm, 149 Å) were purchased from Waters Corporation
(Milford, USA). Nicotinamide adenine dinucleotide phosphate (NADPH)
and MgCl₂ were purchased from Shanghai Macklin Biochemical
Co., Ltd. (Shanghai, China). Six-well plates were obtained from Corning
Incorporated-Life Science (Jiangsu, China).

Wang H., Xu J., Dong P., Li Y., Cui Y., Li H., Li H., Zhang J., Wang S, & Dai L. (2022). Comprehensive Analysis of Pterostilbene Metabolites In Vivo and In Vitro Using a UHPLC-Q-Exactive Plus Mass Spectrometer with Multiple Data-Mining Methods. ACS Omega, 7(43), 38561-38575.

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