Avanti j high speed centrifuge

Soluble Starch (1% w/v, Merck, Cas # 9005-84-9) was added to a 1000 mL⁻¹ Erlenmeyer flask in an modified Melin–Norkrans (MMN) broth with the composition: (g L⁻¹): glucose 1.0; yeast extract 3.0; malt extract 1.0; (NH₄)₂HPO₄ 0.25; KH₂PO₄ 0.50; MgSO₄·7H₂O 0.15; CaCl₂ 0.05; NaCl 0.025; thiamine-HCl 100 µg L⁻¹, ZnSO₄·7H₂O 0.003, and 1.2 mL of FeCl₃ (1% w/v). Production medium was thoroughly mixed, and a 250 mL was placed in a 500 mL shake flask. The medium was sterilized at 121 °C for 15 min and was inoculated with five discs of 5 mm mycelial plugs of the fungus. The uninoculated medium was used as a control. Growth was allowed to proceed at 28 °C in the dark for 3 weeks on a rotary incubator shaker at 150 rpm. After 42 days of growth, the cultures were homogenised using IKA’s ULTRA-TURRAX homogeniser (20,000 rpm), and centrifugation was performed at 10,000×g for 15 min to obtain crude enzyme filtrates (supernatant) using a Beckman Coulter Avanti-J high-speed centrifuge.