Anti-Gpx4 (Abcam, ab41787, 1:2000), Anti-xCT (Abcam, ab37185, 1:2000), Anti-Cas9 (Cell signaling, 14697, 1:1000,), Anti-ß-Actin (Sigma, A1978, 1:10,000), Anti-GCLC (Santa Cruz, sc-166345, 1:1000), Anti-Ascl1 (BD Pharmingen, 556604, 1:1000), Anti-Txnrd1 (Cell signaling, 15140S, 1:1000), Anti-Txnrd2 (Cell signaling, 12029, 1:1000), Anti-Acsl4 (Santa Cruz Biotechnology, sc-271800, 1:2000), Anti-Txn1 (Cell signaling, 2429S, 1:1000), Anti-GAPDH (Cell signaling, 97166S, 1:2000), Anti-FSP1 (previously described55 , kindly provided by M. Conrad, undiluted hybridoma supernatant), Anti-REST1 (ThermoFisher, BS-2590R, 1:1000), Anti-REST1 (Abcam, ab21635, 1:1000), Anti-TXNIP (Cell signaling, 14715S, 1:1000), Anti-CD71 (Santa Cruz, sc-65882, 1:2000), Anti-cMyc (Abcam, ab32072, 1:2000), Anti- NCAM (Invitrogen, PA5-79717, 1:1000), Anti-Vimentin (Abcam, ab137321, 1:1000), Anti-YAP1 (Cell signaling, #4912, 1:1000), Anti-Synatophysin (Invitrogen, MA5-14532, 1:1000), Anti-AGPAT2 (Thermo Fisher, PA5-76010, 1:2000), Anti-AGPAT3 (Thermo Fisher, PA5-101343, 1:2000). HRP-conjugated secondary antibodies: goat-anti-mouse-HRP (Linaris GmBH, 20400-1 mg, 1:10,000), goat-anti-rabbit-HRP (Linaris GmBH, 20402-1 mg, 1:10,000), goat-anti-rat-HRP (Sigma, A9037-1 ml, 1:10,000).
Anti ascl1
Manufactured by BD
Anti-ASCL1 is a laboratory reagent that can be used to detect the presence of the ASCL1 protein in biological samples. ASCL1 is a transcription factor involved in the regulation of cell differentiation. The Anti-ASCL1 reagent can be used in various research applications, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of ASCL1 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti yap1, by Cell Signaling Technology (2 mentions)
Anti neurod1, by Abcam (2 mentions)
Qubit fluorometer, by Thermo Fisher Scientific (2 mentions)
Anti xct, by Abcam (1 mentions)
Anti cas9, by Cell Signaling Technology (1 mentions)
Anti cd71, by Santa Cruz Biotechnology (1 mentions)
Anti ncam, by Thermo Fisher Scientific (1 mentions)
Goat anti rat hrp, by Merck Group (1 mentions)
Anti acsl4, by Santa Cruz Biotechnology (1 mentions)
Anti gpx4, by Abcam (1 mentions)
Anti gclc, by Santa Cruz Biotechnology (1 mentions)
Anti txnip, by Cell Signaling Technology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti c myc, by Abcam (1 mentions)
Anti vimentin, by Abcam (1 mentions)
Anti pcna, by Merck Group (1 mentions)
Anti sox2, by Cell Signaling Technology (1 mentions)
Rabbit anti s100β, by Abcam (1 mentions)
Anti fabp7, by Merck Group (1 mentions)
5 protocols using anti ascl1
1
Comprehensive Immunoblotting Antibody Panel
2
Comprehensive Immunostaining Panel for Neural Cell Types
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies for immunostaining included chicken anti-GFP (1:1000 dilution, Abcam, Cat# ab13970), rat anti-GFP (1:1000, Nacalai Tesque, Cat# GF090R), anti-Sox2 (1:200, Cell Signaling Technology, Cat# 3728), anti-RFP (1:1000, MBL, Cat# PM005), anti-Fabp7 (BLBP, 1:500, Millipore, Cat# ABN14), mouse anti-S100β (1:200, Sigma-Aldrich, Cat# S2657), rabbit anti-S100β (1:500, Abcam, Cat# ab52642), anti-NICD1 (1:200, Cell Signaling Technology, Cat# 4147), anti-GFAP (1:1000, Abcam, Cat# ab4674), anti-Hey1 (1:200, Millipore, Cat# AB5714), anti-Ascl1 (1:200, BD Biosciences, Cat# 556604), anti-PCNA (1:500, Millipore, Cat# NA03), anti-Dcx (1:1000, Abcam, Cat# ab18723), anti-EGFR (1:500, Fitzgerald, Cat# 20-ES04), anti-Vcam1 (1:500, BD Biosciences, Cat# 550547), Hoechst 33342 (1:10000, Molecular Probes).
3
Western Blot Analysis of Transcription Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein lysates were prepared by washing cells with 1 ml of cold PBS and resuspended in lysis buffer [150 mM NaCl, 50 mM tris-HCl (pH 8.0), 1% NP-40, 0.5% Na deoxycholate, 0.1% SDS, and protease inhibitors] for 30 min at 4°C. Lysates were centrifuged at 5°C for 15 min at 13,000 rpm to remove insoluble debris. Protein concentrations were quantified using Pierce BCA (Thermo Fisher Scientific). Proteins were separated by electrophoresis on an SDS–polyacrylamide gel electrophoresis gel (Bio-Rad), transferred to a polyvinylidene difluoride membrane (Thermo Fisher Scientific), and blocked with 5% milk in tris-buffered saline (TBS) with Tween 20. The membranes were immunoblotted with anti–c-Myc (Santa Cruz Biotechnology, sc-40), anti–L-Myc (University of Iowa, PCRP-MYCL1-1A3), anti-vinculin (Sigma-Aldrich), anti-ASCL1 (BD Biosciences, #556604), anti-NeuroD1(Proteintech, 12081-1-AP), anti-Smad2 (Cell Signaling Technology, #5339), anti-Rest (Millipore Sigma, 07-579), anti-Hes1 (Santa Cruz Biotechnology, sc-166410), anti-Brn2 (Cell Signaling Technology, 12137), anti-YAP1 (Proteintech, 13584-1-AP). Immunoblots were quantified using ImageJ.
4
Protein Extraction and Immunoblot Analysis
5
Western Blot Analysis of Cell Lysates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were lysed in RIPA buffer (150 mM NaCl, 10 mM Tris-HCl pH 7.2 0.1% SDS, 1.0% Triton X-100, 1% sodium deoxycholate, and 5 mM EDTA). Protein concentrations were determined with the Qubit™ Fluorometer (Thermo Fisher Scientific). Proteins were subjected to gel electrophoresis and Western blotting (WB) as previously described, 51 using the following antibodies: anti-MYC (1:1000), anti-BCL2 (1:500), and anti-YAP1 (1:1000) (Cell Signaling Technology), anti-POU2F3 (1:1000) (Merck KGaA), anti-NEUROD1
(1:1000) (Abcam), and anti-ASCL1 (1:500) (BD Biosciences).
Immuno-reactive bands were detected with anti-mouse and antirabbit secondary antibodies conjugated to horseradish peroxidase (HRP) obtained from Santa Cruz Biotechnologies. Signals were detected using Clarity and Clarity Max ECL Western Blotting Substrates (Bio-Rad Laboratories) with Chemidoc System (Bio-Rad Laboratories). Some blots were re-tested to detect a second protein after stripping the revealed antibodies using Restore Western Blot stripping Buffer (Thermo Fisher Scientific). Bands were normalized to total proteins using stain-free technology 52 (Bio-Rad Laboratories). Densitometry analysis was performed using ImageLab (Bio-Rad Laboratories).
(1:1000) (Abcam), and anti-ASCL1 (1:500) (BD Biosciences).
Immuno-reactive bands were detected with anti-mouse and antirabbit secondary antibodies conjugated to horseradish peroxidase (HRP) obtained from Santa Cruz Biotechnologies. Signals were detected using Clarity and Clarity Max ECL Western Blotting Substrates (Bio-Rad Laboratories) with Chemidoc System (Bio-Rad Laboratories). Some blots were re-tested to detect a second protein after stripping the revealed antibodies using Restore Western Blot stripping Buffer (Thermo Fisher Scientific). Bands were normalized to total proteins using stain-free technology 52 (Bio-Rad Laboratories). Densitometry analysis was performed using ImageLab (Bio-Rad Laboratories).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!