Bs 4963r

Manufactured by Bioss Antibodies

Sourced in United States

The Bs-4963R is a laboratory instrument designed for performing antibody-based assays. It is a compact and versatile equipment that can be utilized in various research and diagnostic applications. The core function of the Bs-4963R is to facilitate the detection and quantification of target analytes through immunological techniques. This product provides reliable and consistent results, making it a valuable tool for researchers and scientists.

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Lab products found in correlation

Ab76291, by Abcam (1 mentions) Ab134155, by Abcam (1 mentions) Streptavidin conjugation kit, by Abcam (1 mentions) Bs 0756r, by Bioss Antibodies (1 mentions) Alexa fluor 488 labeled anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti nanog, by Abcam (1 mentions) Anti oct4, by Abcam (1 mentions) Anti sox2, by Novus Biologicals (1 mentions) Nb110 37235, by Novus Biologicals (1 mentions) Anti c myc, by Bioss Antibodies (1 mentions) Anti klf4, by Bioss Antibodies (1 mentions) Bs 1064r, by Bioss Antibodies (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

2 protocols using bs 4963r

Antibody Modification and DNA Conjugation

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Fourteen antibodies, including antibodies specific to c-Myc (bs-4963R,
Bioss Inc., US), vimentin (bs-0756R, Bioss Inc., US), Oct4 (bs-1111R, Bioss
Inc., US), POLRMT (489004 Pab, USBiological, US), PARP1 (LS-C745005, LifeSpan
Biosciences Inc., US), ARV7 splice variant antibody (31-1109-00, RevMAb
Biosciences, US), BCR-ABL1 (ab187831, Abcam, US), BRAFv600E (31-1042-00, RevMAb
Biosciences, US), TrkA (ab76291, Abcam, US), TrkB (ab134155, Abcam, US), BRCA2
(N-terminus specific, ab75335, Abcam, US), and BRCA2 (C-terminus specific,
ARG10523, Arigo Biolaboratories Corp., US), pan cytokeratin (bs-1712R, Bioss
Inc., US), and KRAS 2B (16155-1-AP, Proteintech, US) were first modified with
streptavidin using a streptavidin conjugation kit (Abcam, US), according to the
manufacturer’s protocol. Briefly, 100 μL of the antibody solution
(1 mg mL⁻¹) were gently shaken with 10 μL of the
activator. Afterward, the activated antibodies were incubated with 33 μg
streptavidin overnight at 4°C. Next, 10 μL of the quencher were
added to stop the reaction. Then, 80 μL of the biotin-labeled DNA (1 mg
mL⁻¹) were added and the mixture was incubated at room
temperature for 30 min. Finally, the solution was stored at 4°C until
use.

Labib M., Wang Z., Ahmed S.U., Mohamadi R.M., Duong B., Green B., Sargent E.H, & Kelley S.O. (2020). Tracking the expression of therapeutic protein targets in rare cells via antibody-mediated nanoparticle labelling and sorting. Nature biomedical engineering, 5(1), 41-52.

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Immunofluorescence Staining of Stem Cell Markers

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The cells were rinsed briefly with phosphate-buffered saline (PBS) and fixed for 20 min in 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) at room temperature. The cells were permeabilized for 10 min with 0.1% Triton X-100 in PBS, and blocked for 45–60 min with 4% bovine serum albumin in PBS at room temperature. Cells were incubated overnight at 4 °C with one of the following antibodies: anti-Oct4 (1:500; Abcam, Cambridge, MA, USA), anti-Sox2 (1:500; NB110-37235, Novus Biologicals, Littleton, CO, USA), anti-Nanog (1:500; Abcam, Cambridge, MA, USA), anti-c-Myc (1:250; bs-4963R, Bioss, Woburn, MA, USA), anti-Klf4 (1:250, bs-1064R, Bioss, Woburn, MA, USA). This was followed by incubation with the following secondary antibody: Alexa Fluor 488-labeled anti-rabbit IgG (1:500; Invitrogen, Carlsbad, CA, USA). Nuclei were counterstained using DAPI (1 mg/mL PBS; Invitrogen, Carlsbad, CA, USA).

El-Sayed A.K., Zhang Z., Zhang L., Liu Z., Abbott L.C., Zhang Y, & Li B. (2014). Pluripotent State Induction in Mouse Embryonic Fibroblast Using mRNAs of Reprogramming Factors. International Journal of Molecular Sciences, 15(12), 21840-21864.

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