Stim1

Manufactured by Abcam

Sourced in United Kingdom, United States

STIM1 is a calcium sensor protein that plays a key role in the regulation of calcium homeostasis within cells. It is involved in the process of store-operated calcium entry (SOCE), which is a mechanism that allows the replenishment of calcium stores in the endoplasmic reticulum (ER) from extracellular sources.

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Lab products found in correlation

Orai1, by Abcam (2 mentions) Phospho paxillin tyr118, by Cell Signaling Technology (1 mentions) Vinculin, by Santa Cruz Biotechnology (1 mentions) Phospho myosin light chain 2, by Cell Signaling Technology (1 mentions) Thiamet g, by Bio-Techne (1 mentions) O glcnac, by Abcam (1 mentions) Pugnac, by Abcam (1 mentions) Skf96365, by Bio-Techne (1 mentions) Mg132, by Cell Signaling Technology (1 mentions) 2 apb, by Bio-Techne (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions) Thapsigargin, by Thermo Fisher Scientific (1 mentions) Cyclopiazonic acid, by Merck Group (1 mentions) D luciferin, by Merck Group (1 mentions) Alpha smooth muscle actin, by Abcam (1 mentions) β actin, by Merck Group (1 mentions) Cytokeratin 8, by Abcam (1 mentions) Cytokeratin 18, by Abcam (1 mentions) Hsc70, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-STIM1 (12 citations) Anti-STIM1 antibody (2 citations)

4 protocols using stim1

Western Blot Protein Analysis Protocol

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Western blots were performed as previously described56 (link). The following antibodies were used: MCU (Abcam #Ab121499; dilution: 1/500), F₁F₀ ATPase (BD #612518; dilution: 1/1000), Vinculin (Santa Cruz #sc-55465; dilution: 1/2000), Paxillin H114 (Santa Cruz #sc-5574; dilution: 1/1000), Phospho-Paxillin Tyr 118 (Cell Signaling Technology #2541; dilution: 1/1000), Phospho-Myosin light chain 2 (Cell Signaling Technology #3671; dilution: 1/1000), STIM1 (Abcam #Ab57834; dilution: 1/1000) and Rac1 (Cell Biolabs #240106; dilution: 1/1000).

Prudent J., Popgeorgiev N., Gadet R., Deygas M., Rimokh R, & Gillet G. (2016). Mitochondrial Ca2+ uptake controls actin cytoskeleton dynamics during cell migration. Scientific Reports, 6, 36570.

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Investigating TRPM7 and O-GlcNAcylation

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2-APB, SKF96365, and thiamet G were obtained from Tocris Bioscience (Bristol, UK). PugNAc and antibodies for TRPM7, ORAI1, STIM1, OGA, O-GlcNAc and ITGB7 were obtained from Abcam (Cambridge, UK). Antibody for ubiquitin was from Santa Cruz Biotechnology (Dallas, TX, USA) and secondary antibodies were from EMD Millipore (Berlington, MA, USA). MG-132 and all other antibodies were from Cell Signaling Technology (Beverly, MA, USA). Other reagents were from Sigma-Aldrich (Dallas, MA, USA).

Samart P., Luanpitpong S., Rojanasakul Y, & Issaragrisil S. (2021). O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination. Journal of Experimental & Clinical Cancer Research : CR, 40, 100.

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Antibody Screening for Immunoblotting and Immunohistochemistry

Cited 1 time

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Antibodies used for immunoblotting were as follows: HSC70 (# sc-7298) and TRPC1 (# sc-133076) from Santa Cruz Biotechnology; STIM1 (# ab57834) from Abcam; Orai1 (# O8264) and beta-actin (# SAB1305567) from Millipore-Sigma; Caveolain-1 (#610057) from BD Biosciences. In-house generated Protein G-purified rabbit polyclonal rabbit anti-EHD2 antisera has been described previousl (George et al., 2007 (link)). The horseradish peroxidase (HRP)-conjugated Protein A or HRP-conjugated rabbit anti-mouse secondary antibody for immunoblotting were from Invitrogen. The alpha smooth muscle actin (# ab7817), cytokeratin 8 (#53280), cytokeratin 18 (# 133263), Ki67 (# ab16667) antibodies for immunohistochemistry (IHC) and immunofluorescence (IF) staining were from Abcam. Thapsigargin (# T7459) and Fluo 4AM (# 14201) were from Thermo Fisher Scientific. Cyclopiazonic acid (# C1530) and D-luciferin (#L9504) were from Millipore Sigma. SKF96365 (cat. # S7999), SOCE inhibitor CM4620 (# S6834) from SelleckChem, Matrigel (# 356230) from Corning, and Isoflurane (# 502017) from MWI Animal Health.

Luan H., Bielecki T.A., Mohapatra B.C., Islam N., Mushtaq I., Bhat A.M., Mirza S., Chakraborty S., Raza M., Storck M.D., Toss M.S., Meza J.L., Thoreson W.B., Coulter D.W., Rakha E.A., Band V, & Band H. (2023). EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry. eLife, 12, e81288.

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Protein Expression Analysis of Key Cellular Pathways

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Antibodies used in the study were purchased form Abcam (Cambridge, UK) and Cell Signaling Technology (Danvers, MA, USA): actin (CST, #3700); SULT1A2 (Abcam, ab123976); PRSS1 (Abcam, ab200996); INTS4 (Abcam, ab75253); PFDN1 (Abcam, ab151708); CIAPIN1 (Abcam, ab154904); FASN (CST, #3180); ACACA (CST, #3662); STIM1 (Abcam, ab57834); TFRC (Abcam, ab84036); FTL (Abcam, ab218400); FTH (Abcam, ab77127); FLT3 (CST, #3462); pFLT3 (CST, #4577); STAT5 (CST, #25656); pSTAT5 (CST, #4322); PTPRJ (Abcam, ab181244); GPX1 (CST, #3206).

Chen X., Zhang J., Yuan L., Lay Y., Wong Y.K., Lim T.K., Ong C.S., Lin Q., Wang J, & Hua Z. (2017). Andrographolide Suppresses MV4-11 Cell Proliferation through the Inhibition of FLT3 Signaling, Fatty Acid Synthesis and Cellular Iron Uptake. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(9), 1444.

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