Sodium alginate with an average molecular weight of 280kDa (high-MW) was obtained for these studies (LF20/40, FMC Biopolymer). Molecular weight of alginate was modulated as has been described previously18 (link). Briefly, Mid- or low-MW alginate was prepared by irradiating high-MW alginate with a 3 or 8 Mrad cobalt source, respectively. mid-MW alginate had an average molecular weight of 70 kDA and low-MW alginate had an average molecular weight of 35 kDa. Polyethylene glycol (PEG) coupled alginate was prepared following a previous protocol18 (link). Briefly, one chain of low-MW alginate was covalently coupled to an average of two PEG-amine molecules (5 kDa, Laysan Bio) using carbodiimide chemistry. Specifically, at a pH of 6.5, 500 mg of alginate was dissolved in 50 ml of 0.1 M MES (2-(N -morpholino) ethanesulfonic acid, Sigma-Aldrich) buffer. Then 13.7 mg of Sulfo-NHS, 24.2 mg of EDC (N -(3-dimethylaminopropyl)-N0 -ethylcarbodiimide hydrochloride, Sigma-Aldrich), and 295 mg of PEG-amine was mixed with the solution, and the reaction was allowed to proceed for 20 h. The resulting solution was dialyzed for three days in deionized water (molecular weight cutoff of 10 kDa). Finally, the product was purified with activated charcoal, sterile filtered, frozen and lyophilized.
N 3 dimethylaminopropyl n 0 ethylcarbodiimide hydrochloride
Manufactured by Merck Group
Sourced in United Kingdom
N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is a water-soluble carbodiimide that is commonly used as a coupling agent in organic synthesis and biochemistry. It facilitates the formation of amide bonds between carboxyl and amine groups.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide, by Merck Group (4 mentions)
Peg amine molecules, by Laysan Bio (2 mentions)
0.1 m mes 2 n morpholino ethanesulfonic acid, by Merck Group (2 mentions)
Lf20 40, by FMC Biopolymer (2 mentions)
Sulfo nhs, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Poly ethylene glycol monomethyl ether, by Merck Group (1 mentions)
Fecl2 4h2o, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Fecl3 6h2o, by Merck Group (1 mentions)
Triethylamine tea, by Merck Group (1 mentions)
Benzalkonium chloride, by Merck Group (1 mentions)
Calcium chloride dihydrate, by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Sodium bicarbonate, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Polyethylene glycol sorbitan monolaurate tween 20, by Merck Group (1 mentions)
Nile red, by Merck Group (1 mentions)
9 protocols using n 3 dimethylaminopropyl n 0 ethylcarbodiimide hydrochloride
1
Engineered Alginate Hydrogel Synthesis
2
Synthesis of PEGylated Iron Oxide Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FeCl3.6H2O, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), FeCl2.4H2O, Polyethylene glycol monomethyl ether (mPEG, MW = 4 kDa), ammonium hydroxide, N-(3-Dimethylaminopropyl)-N0-ethyl-Carbodiimide hydrochloride (EDC) succinic anhydride, Dimethylsulfoxide (DMSO), N-Hydroxy Succinimide (NHS), dimethyl amino pyridine (DMAP), N, N′-dicyclohexyl anhydrous 1,4-dioxane, and triethylamine (TEA) were obtained from Sigma Aldrich. All other chemicals were purchased from Emertat Chimi Company (Tehran, Iran).
3
Engineered Alginate Hydrogel Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium alginate with an average molecular weight of 280kDa (high-MW) was obtained for these studies (LF20/40, FMC Biopolymer). Molecular weight of alginate was modulated as has been described previously18 (link). Briefly, Mid- or low-MW alginate was prepared by irradiating high-MW alginate with a 3 or 8 Mrad cobalt source, respectively. mid-MW alginate had an average molecular weight of 70 kDA and low-MW alginate had an average molecular weight of 35 kDa. Polyethylene glycol (PEG) coupled alginate was prepared following a previous protocol18 (link). Briefly, one chain of low-MW alginate was covalently coupled to an average of two PEG-amine molecules (5 kDa, Laysan Bio) using carbodiimide chemistry. Specifically, at a pH of 6.5, 500 mg of alginate was dissolved in 50 ml of 0.1 M MES (2-(N -morpholino) ethanesulfonic acid, Sigma-Aldrich) buffer. Then 13.7 mg of Sulfo-NHS, 24.2 mg of EDC (N -(3-dimethylaminopropyl)-N0 -ethylcarbodiimide hydrochloride, Sigma-Aldrich), and 295 mg of PEG-amine was mixed with the solution, and the reaction was allowed to proceed for 20 h. The resulting solution was dialyzed for three days in deionized water (molecular weight cutoff of 10 kDa). Finally, the product was purified with activated charcoal, sterile filtered, frozen and lyophilized.
4
Surface Plasmon Resonance Analysis of TRAIL-DR5 Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Interactions of TRAIL or TRAIL-ATNC with TRAIL receptor, DR5, were analyzed using a surface plasmon resonance instrument (SR7500 DC, Reichert Inc., NY, USA) as described previously43 (link). The extracellular region (1–182) of the DR5 protein (10465-H08H, Sino Biological, Beijing, China) was immobilized by activating the carboxymethyl group on dextran-coated chips through a reaction with a mixture of N-(3-dimethylaminopropyl)-N0-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (Sigma-Aldrich, St. Louis, MO, USA). Different concentrations of TRAIL-ATNC (0.52 to 8.33 nM) and TRAIL (25 to 400 nM) in binding buffer (100 mM Tris pH 7.4, 150 mM NaCl, 0.005% Tween20, 1 mM DTT) were allowed to flow over surfaces containing immobilized DR5 (585 RU) at a rate of 25 μl/min at 25 ℃. The sensor surface was regenerated after each association and dissociation cycle by injecting 2 M NaCl and 10 mM HCl for 1 min and 15 s, respectively. Sensorgrams were fit to a simple 1:1 Langmuir interaction model using data analysis program Scrubber 2.0 (BioLogic Software, Australia).
5
Biomimetic Nanoparticle Emulsion Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oligoglycine Gly 4 -NH-C 10 H 20 -NH-Gly 4 (2T, purity 4 95%) was purchased from PlasmaChem GmbH (Berlin, Germany) and used as received. Monodispersed, single-digit, carboxylated detonation ND (nominally 5 nm diameter, with a concentration of 10 mg mL À1 in water), N-(3-dimethylaminopropyl)-N 0 -ethyl carbodiimide hydrochloride (EDC, 99%), N-hydroxysuccinimide (NHS, 98%), benzalkonium chloride (BAC), and Nile Red (for microscopy in retention tests) were used as received from Sigma-Aldrich (Merck Life Science UK Limited; Gillingham, UK). Pure sunflower oil (Florar, Princes Ltd, UK) was used as the oil phase (without any purification) to prepare o/w emulsions. Polyethylene glycol sorbitan monolaurate (Tween 20, product number P1379, Sigma-Aldrich) was used in comparative emulsions as a surfactant. Sodium chloride, sodium bicarbonate, calcium chloride dihydrate, and phosphate-buffered saline (PBS) tablets were purchased from Fisher Scientific UK Ltd (Loughborough, United Kingdom). Aqueous solutions of HCl and NaOH (0.1 M, purchased from Sigma-Aldrich) and deionised (DI) water (18.2 MO cm, Elga DI water system) were used in experiments.
6
Immobilization of Peptides on Polymer Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetylcholineesterase, from electric eel Electrophorus electricus (EeAChE), bovine pancrease trypsin, tacrine, malathion, N-isopropylacrylamide (NIPAm), N,N 0 -methylenebisacrylamide (BIS), N-tert-butylacrylamide (TBAm), acrylic acid (AAc), N-(3-aminopropyl)methacrylamide (APMA), phosphate buffered saline (PBS), ammonium persulfate (APS), N,N,N 0 ,N 0 -tetramethylethylenediamine (TEMED), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N 0 -ethylcarbodiimide hydrochloride, ethanolamine, sodium hydroxide, sulphuric acid, acetone, methanol, acetonitrile, toluene, (3-aminopropyl)triethoxysilane, 1,2-bis(triethoxysilyl)ethane, succinimidyl iodoacetate (SIA), ethylenediaminetetraacetic acid (EDTA), formic acid were purchased from Sigma-Aldrich, UK.
The peptides (CLALQWVQDNIHFFGGNPK, CQVTIFGESA-GAASVGMHLLSPDSRPK, CFRFSFVPV and CYWANFAR) were custom-made by Zhejiang Ontores Biotechnologies Co., Ltd (China). The cysteine residue was added to the carboxyl end of each peptide for immobilization using thiol coupling.
The peptides (CLALQWVQDNIHFFGGNPK, CQVTIFGESA-GAASVGMHLLSPDSRPK, CFRFSFVPV and CYWANFAR) were custom-made by Zhejiang Ontores Biotechnologies Co., Ltd (China). The cysteine residue was added to the carboxyl end of each peptide for immobilization using thiol coupling.
7
Tyramine-Functionalized Hyaluronic Acid for Tissue Engineering
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium hyaluronate (MW = 70 kDa) was purchased from HTL SAS (Javené, France) and used without further purification. Tyramine (TA) (99%), anhydrous N,N-dimethylformamide (DMF) (99.8%), N-(3-dimethylaminopropyl)-N 0 -ethylcarbodiimide hydrochloride (EDAC), N-hydroxysuccinimide (NHS), hydrogen peroxide (H 2 O 2 ), and horseradish peroxidase (HRP, 325 units/mg solid), Hyaluronidase from bovine testes (lyophilized powder, 400-1000 units/mg), MES hemisodium salt dry powder, were obtained from Sigma-Aldrich and used without further purification. Phosphate buffered saline (PBS, 10 mM, pH 7.4) was purchased from Lonza. All other solvents were purchased from Biosolve (Valkenswaard, The Netherlands) and used as received. Platelet lysate and Heparin sodium salt solution (5000 U/ml) were purchased from PL BioScience (Aachen, Germany)
8
Synthesis and Characterization of Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bovine serum albumin (fatty acid free), Tempo-4-amino (97%), 4-amino salicylic acid (99%), 4-amino benzoic acid (99%), 3-amino phenol (98%), phenyl isothiocyanate (97%), cyclohexyl isothiocyanate (98%), hexyl isothiocyanate (95%), ibuprofen (98%), acetyl salicylic acid (99%), N-(3-dimethylaminopropyl)-N 0 -ethylcarbodiimide hydrochloride (EDC, 98%), hydrochloric acid (37%, 12.2 M), dichloromethane (99.8%), trimethylamine (99.5%), ethyl acetate (99.5%), methanol (99.8%) were purchased from Sigma-Aldrich. Thiophosgene (99%) was purchased from Merck.
9
Aflatoxin M1 Immunoassay Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were carried out with analytical-grade chemical reagents used as received without further purification. Deionized ultrapure water (18.2 MU/cm) was obtained with a Milli-Q system (Millipore, Bedford, MA, USA). Zinc sulfate heptahydrate, manganese chloride tetrahydrate, L-cysteine hydrochloride monohydrate, and standard solutions of 1000 mg L -1 of Mn, Zn and S were obtained from MERCK (Darmstadt, Germany). Sodium sulfide nonahydrate, sodium hydroxide, lipoic acid, potassium tert-butoxide, N-(3-Dimethylaminopropyl)-N0-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), TWEEN 20, bovine serum albumin (BSA), aflatoxin M1 BSA conjugate, free aflatoxin M1 and methanol HPLC gradient grade were purchased from Sigma-Aldrich (Schnelldorf, Germany). Rat monoclonal anti-aflatoxin and goat polyclonal anti-rat antibody were obtained from Abcam (Cambridge, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!