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Berberine phr1502

Manufactured by Merck Group
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Sourced in United States

Berberine (PHR1502) is a laboratory product offered by Merck Group. It is a naturally occurring compound with molecular formula C20H18NÅ. This product is intended for research use only and its core function is as a chemical standard.

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Lab products found in correlation

Glibenclamide, by Merck Group (1 mentions) Tolbutamide, by Merck Group (1 mentions) Verapamil, by Merck Group (1 mentions) Mdivi 1 ab144589, by Abcam (1 mentions) D glucose g7528, by Merck Group (1 mentions) P mtor ser 2481, by Abcam (1 mentions) Compound c ab120843, by Abcam (1 mentions) Pvdf membrane, by GE Healthcare (1 mentions) Pgc 1α, by ABclonal (1 mentions) Ab39012, by Abcam (1 mentions) Ab41037, by Abcam (1 mentions) Ab3778, by Abcam (1 mentions) Ab34712, by Abcam (1 mentions) Cell counting kit 8 ck04, by Dojindo Laboratories (1 mentions) Fetal bovine serum fbs 10099 141, by Thermo Fisher Scientific (1 mentions) Ab8245, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole dapi c1002, by Beyotime (1 mentions)

3 protocols using berberine phr1502

1

Hyperglycemic Clamp Study of Berberine

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Tolbutamide, glibenclamide, and verapamil were purchased from Sigma–Aldrich (St. Louis, MO, USA). Berberine (PHR1502, Lot#LRAA9232) used in the animal and in vitro studies was purchased from Sigma–Aldrich. For the hyperglycemic clamp study, tablets of BBR (Lot#23180501) and the placebo (Lot#180401) used in human subjects were admitted by the State Food and Drug Administration (SFDA) of China and were obtained from Xin Yi Tian Ping Pharmaceutical Co., Ltd. (Shanghai, China). Antibodies used for immunoblotting and immunofluorescence staining are listed in Supplementary Table 3.
Zhao M.M., Lu J., Li S., Wang H., Cao X., Li Q., Shi T.T., Matsunaga K., Chen C., Huang H., Izumi T, & Yang J.K. (2021). Berberine is an insulin secretagogue targeting the KCNH6 potassium channel. Nature Communications, 12, 5616.
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2

Mitochondrial Dynamics and Autophagy Regulation

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Following monoclonal antibodies were purchased from CST: Mfn1 (#14739), Mfn2 (#9482), OPA1 (#67589), Drp1 (#8570), p-Drp1 Ser 616 (#4494), MFF (#84580), Beclin1 (#3495), Atg5 (#12994), p62 (#23214), LC3A/B (#12741), mTOR (#2983), Parkin (#4211), AMPKα (#5831), and p-AMPK Thr172α (#2535). Following monoclonal antibodies were purchased from Abcam: β-actin (ab8826), PINK1 (ab75487), and p-mTOR Ser 2481 (ab137133). Following polyclonal antibodies were purchased from ABclonal: VDAC (A15735) and PGC1α (A12348). All of these above antibodies were used at 1:1000 dilution, besides β-actin (ab8826) at 1:5000 and PINK1 (ab75487) at 1:200. PVDF membrane was purchased from GE Health (10600023). D-Glucose (G7528) and berberine (PHR1502) were purchased from Sigma, Mdivi-1(ab144589) and compound C (ab120843) were purchased from Abcam.
Hang W., He B., Chen J., Xia L., Wen B., Liang T., Wang X., Zhang Q., Wu Y., Chen Q, & Chen J. (2018). Berberine Ameliorates High Glucose-Induced Cardiomyocyte Injury via AMPK Signaling Activation to Stimulate Mitochondrial Biogenesis and Restore Autophagic Flux. Frontiers in Pharmacology, 9, 1121.
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3

Berberine's Chondroprotective Effects

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Berberine (PHR1502), tert-butyl hydroperoxide (TBHP) (458139), dimethyl sulphoxide (DMSO) (D2650), 3-methyladenine (3-MA) (M9281) and type II collagenases (1148090) were purchased from Sigma-Aldrich (St Louis, MO, USA). Cell-Counting Kit-8 (CCK-8) (CK04) was purchased from Dojindo (Kumamoto, Japan). Primary antibodies against Collagen II (ab34712), Aggrecan (ab3778), MMP-13 (ab39012), ADAMTS-5 (ab41037), Bax (ab32503), Bcl-2 (ab59348), Cytochrome C (ab90529), P62 (ab56416) and GAPDH (ab8245) were purchased from Abcam (Cambridge, MA, USA). LC3 (12741S), Beclin-1 (3495S), ATG7 (8558S) and Cleaved-caspase3 (9664S) antibodies were acquired from CST (Beverly, MA, USA). Dulbecco's modified Eagle's medium (DMEM)/F12 (11320082), and fetal bovine serum (FBS) (10099141) were purchased from Gibco (Grand Island, NY, USA). FITC-labeled and horseradish peroxidase-labeled secondary antibodies (BS10000, BS13278) were purchased from Bioworld (MN, USA). 4', 6-diamidino-2-phenylindole (DAPI) (C1002) was obtained from Beyotime (Shanghai, China).
Chen Y., Zheng Z., Wang J., Tang C., Khor S., Chen J., Chen X., Zhang Z., Tang Q., Wang C., Lou Y., Wang Z., Xiao J, & Wang X. (2018). Berberine suppresses apoptosis and extracellular matrix (ECM) degradation in nucleus pulposus cells and ameliorates disc degeneration in a rodent model. International Journal of Biological Sciences, 14(6), 682-692.
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