Hematoxylin qs counterstain

Manufactured by Vector Laboratories

Hematoxylin QS counterstain is a laboratory product designed for use in histological and cytological staining procedures. It serves as a counterstain, providing a contrasting color to the primary stain in order to enhance the visualization of cellular and tissue structures.

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Lab products found in correlation

Mmp 9, by Cell Signaling Technology (1 mentions) Evos xl core microscope, by Thermo Fisher Scientific (1 mentions) Matrigel, by R&D Systems (1 mentions) Eosin y solution, by Merck Group (1 mentions) Bluing buffer, by Agilent Technologies (1 mentions)

Spelling variants of this product

Hematoxylin (236 citations) Hematoxylin QS (145 citations) Hematoxylin counterstain (3 citations)

4 protocols using hematoxylin qs counterstain

Quantitative Lung Tissue Immunohistochemistry

Cited 3 times

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Lung tissue was analyzed for detection of PCNA (2586, Cell Signaling) and MMP-9 (13667, Cell Signaling) as published (Perer et al., 2020 (link); Jandova and Wondrak, 2021 (link)). Nuclear staining was performed using Hematoxylin QS counterstain (H3404; Vector Laboratories, Burlingame, CA). Images were captured using an Olympus BX50 and Spot (Model 2.3.0) camera. Quantitative tissue scoring (H-score) was performed as published (Bair et al., 2010 (link)).

Jandova J, & Wondrak G.T. (2021). Vemurafenib drives EMT gene expression in BRAFi-resistant BRAFV600E/NRASQ61K melanoma enhancing tumor growth and metastasis in a bioluminescent murine model. The Journal of investigative dermatology, 142(5), 1456-1465.e1.

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Cell Migration and Invasion Assay

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In all, 3 × 10⁴ or 5 × 10⁴ cells were suspended in serum-free media in 8-μm transwell inserts (#83.3932.800, Sarstedt), for 24-h migration or 48-h invasion assays, respectively. For the invasion assay, the upper chamber was pre-coated with 1/9 dilution of Matrigel (Cultrex Reduced Growth Factor Basement Membrane Extract, #3433-005-01, R&D Systems). After incubation, inserts were washed with PBS. Migrated or invaded cells on the underside of the membrane were fixed with 4% paraformaldehyde and stained with hematoxylin (Hematoxylin QS Counterstain, #H-3404, Vector Laboratories). Migrated or invaded cells were counted from six representative fields from the inverted EVOS XL core microscope (Thermo Fisher Scientific).

Beniey M., Hubert A., Haque T., Cotte A.K., Béchir N., Zhang X., Tran-Thanh D, & Hassan S. (2023). Sequential targeting of PARP with carboplatin inhibits primary tumour growth and distant metastasis in triple-negative breast cancer. British Journal of Cancer, 128(10), 1964-1975.

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Hematoxylin and Eosin Staining

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Frozen sections were treated with Hematoxylin QS Counterstain (#H-3404-100, Vector Laboratories), followed by three washes in distilled water. Sections were then treated briefly with bluing buffer (#CD702, DAKO), followed by three washes in distilled water. Finally, sections were treated briefly with Eosin Y solution (#HT110116, Sigma) followed by three washes in distilled water.

Jeffery E.C., Mann T.L.A., Pool J.A., Zhao Z, & Morrison S.J. (2022). Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair. Cell stem cell, 29(11).

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Brain Tissue PCNA and MELTF Analysis

Cited 2 times

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Brain tissue was analyzed for detection of proliferating cell nuclear antigen (PCNA) (2586, Cell Signaling Technology) and MELTF (NBP1-85777, Novus Biologicals) as published.^{20 (link),22 (link)} Nuclear staining was performed using Hematoxylin QS counterstain (H3404; Vector Laboratories, Burlingame, CA). Images were captured using an Olympus BX50 and Spot (Model 2.3.0) camera.

Jandova J., Park S.L., Corenblum M.J., Madhavan L., Snell J.A., Rounds L, & Wondrak G.T. (2022). Mefloquine induces ER stress and apoptosis in BRAFi-resistant A375-BRAFV600E/NRASQ61K malignant melanoma cells targeting intracranial tumors in a bioluminescent murine model. Molecular carcinogenesis, 61(6), 603-614.

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