Ortho phosphoric acid

Manufactured by Chempur

Sourced in Poland

Ortho-phosphoric acid is a chemical compound with the formula H3PO4. It is a colorless, odorless, and viscous liquid that is widely used in various industrial and laboratory applications. The primary function of ortho-phosphoric acid is to serve as a common laboratory reagent, where it is employed in titrations, pH adjustments, and the preparation of buffer solutions.

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Lab products found in correlation

Acetonitrile, by Merck Group (7 mentions) Ethyl acetate, by Merck Group (6 mentions) Quercetin, by Merck Group (5 mentions) Chlorogenic acid, by Merck Group (5 mentions) Deionized water, by Merck Group (5 mentions) Methanol, by Merck Group (5 mentions) Quercetin 3 o rutinoside, by Merck Group (5 mentions) Caffeic acid, by Merck Group (4 mentions) Quercetin 3 o glucoside, by Merck Group (4 mentions) Hydrochloric acid (hcl), by Chempur (3 mentions) Acetic acid, by Chempur (3 mentions) Metaphosphoric acid, by Merck Group (3 mentions) Gallic acid, by Merck Group (3 mentions) Kaempferol 3 o glucoside, by Merck Group (3 mentions) Phosphate buffered saline (pbs), by Merck Group (3 mentions) Chlorophyll a, by Merck Group (2 mentions) Chlorophyll b, by Merck Group (2 mentions) Magnesium carbonate, by Chempur (2 mentions) Dehydroascorbic acid, by Merck Group (2 mentions) Methanol, by Chempur (2 mentions)

Close spelling variants of this product

Phosphoric acid (5 citations)

11 protocols using ortho phosphoric acid

Phytochemical Profiling of Natural Compounds

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The following chemical reagents were used in this study: 2′2-azinebis-3-ethylbenzothiazolin-6-sulfonic acid (Merck, Warsaw, Poland), acetic acid (Chempur, Warsaw, Poland), acetonitrile (Sigma-Aldrich, Warsaw, Poland), deionized water (Sigma-Aldrich, Warsaw, Poland), ethyl acetate (Sigma-Aldrich, Warsaw, Poland), hydrochloric acid (Chempur, Warsaw, Poland), magnesium carbonate (Sigma-Aldrich, Warsaw, Poland), methanol (Merck, Warsaw, Poland), ortho-phosphoric acid (Chempur, Warsaw, Poland), and phenolic compound standards, including gallic acid (CAS 149-91-7), chlorogenic acid (CAS 327-97-9), p-coumaric (CAS 501-98-4), caffeic acid (CAS 331-39-5), quercetin-3-O-glucoside (CAS 482-35-9), quercetin-3-O-rutinoside (CAS 207671-50-9), myrycetin (CAS 529-44-2), quercetin (CAS 849061-97-8), cyanidin-3-O-glucoside (CAS 7084-24-4), and cyjanidin-3-O-rutinoside (CAS 18719-76-1), lutein (CAS 127-40-2), zeaxanthin (CAS 144-68-3), β-carotene (CAS 7235-40-7), chlorophyll a (CAS 479-61-8), chlorophyll b (CAS 519-62-0) (Sigma-Aldrich, Supelco, Poland), phosphate buffered saline, and potassium persulfate (Sigma-Aldrich, Warsaw, Poland).

Kozicka M, & Hallmann E. (2023). Identification and Quantification of Bioactive Compounds in Organic and Conventional Edible Pansy Flowers (Viola × wittrockiana) and Their Antioxidant Activity. Plants, 12(6), 1264.

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Extraction and Quantification of Phytochemicals

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Acetone (HPLC grade) came from Sigma-Aldrich (Poznań, Poland); Acetonitrile (HPLC grade) came from Chempur (Piekary Śląskie, Poland); Carotenoids, vitamin C, chlorophylls, and phenolics standards (HPLC grade 99.5–99.9% pure) came from Fluka and Sigma-Aldrich (Poznań, Poland): β-carotene, caffeic acid, chlorogenic acid, chlorophyll a, chlorophyll b, dehydroascorbic acid, ferulic acid, gallic acid, kaempferol-3-O-glucoside, lutein, l-ascorbic acid, p-coumaric acid, quercetin, quercetin-3-O-rutinoside; Ethyl acetate came from Sigma Aldrich (Poznań, Poland); Magnesium carbonate (ultrapure) came from Chempur (Piekary Śląskie, Poland); Meta-phosphoric acid came from Sigma-Aldrich (Poznań, Poland); Methanol (HPLC grade) came from Chempur (Piekary Śląskie, Poland); Ortho-phosphoric acid came from Chempur (Piekary Śląskie, Poland).

Kopczyńska K., Kazimierczak R., Średnicka-Tober D., Barański M., Wyszyński Z., Kucińska K., Perzanowska A., Szacki P., Rembiałkowska E, & Hallmann E. (2020). The Profile of Selected Antioxidants in Two Courgette Varieties from Organic and Conventional Production. Antioxidants, 9(5), 404.

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Antioxidant Capacity Determination Using ABTS

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ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (Sigma-Aldrich, Poland); acetonitrile at HPLC purity (Sigma-Aldrich, Poznan, Poland); deionized water (Sigma-Aldrich, Poland); methanol at HPLC purity (Merck, Poznan, Poland); ortho-phosphoric acid 99.9% (Chempur, Piekary Śląskie, Poland); phenolics standards (purity 99.5–99.9%) of gallic, chlorogenic, p-coumaric benzoic, ellagic, oenothein B, quercetin-3-O-rutinoside, myricetin, luteolin, quercetin, quercetin-3-O-glucoside, and kaempferol (Sigma-Aldrich, Poland); and phosphate-buffered saline (Merck, Poznan, Poland) were used.

Jariene E., Lasinskas M., Danilcenko H., Vaitkeviciene N., Slepetiene A., Najman K, & Hallmann E. (2020). Polyphenols, Antioxidant Activity and Volatile Compounds in Fermented Leaves of Medicinal Plant Rosebay Willowherb (Chamerion angustifolium (L.) Holub). Plants, 9(12), 1683.

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Fabrication of Graphene Oxide Sheets

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Copper sheets (purity: 99.99%, size 10 × 15 mm, thickness: 1 mm, not annealed), graphite powder (325 mesh, purity: 99.9995%, Alfa Aesar, Haverhill, MA, USA), potassium permanganate (KMnO₄, purity: 99.0%, POCH, Gliwice, Poland), orthophosphoric acid (H₃PO₄, 85%, POCH), sulfuric acid (H₂SO₄, 95%, POCH), hydrogen peroxide (H₂O₂, 30%, CHEMPUR, Piekary Slaskie, Poland), ethanol (C₂H₅OH, 96%, POCH), hydrochloric acid (HCl, 35–38%, POCH), acetone ((CH₃)₂CO, POCH), ammonium thiocyanate (NH₄SCN, purity: 99.0%, POCH), and sodium chloride (NaCl, purity: 99.5%, POCH) were used.

Ollik K., Karczewski J, & Lieder M. (2021). Effect of Functionalization of Reduced Graphene Oxide Coatings with Nitrogen and Sulfur Groups on Their Anti-Corrosion Properties. Materials, 14(6), 1410.

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Solvent Characterization for Ibuprofen Extraction

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All reagents were commercially available materials and were used without further purification. (RS)-Ibuprofen (99%) was obtained from Acros Organics (Geel, Belgium). l-Valine (≥99%) was purchased from Carl Roth (Karlsruhe, Germany). Trimethylsilyl chloride (≥99%) (TMSCl) was provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Methanol (MeOH), ethanol (EtOH), propan-2-ol (iPrOH), propan-1-ol (PrOH), butan-1-ol (BuOH), pentan-1-ol (AmOH), hexan-1-ol (HexOH), heptan-1-ol (HeptOH), acetic acid, potassium chloride, sodium chloride, orthophosphoric acid (98%), diethyl ether was high purity obtained from Chempur (Gliwice, Poland). Ammonium hydroxide solution 25% (NH₃·H₂O) was of analytical grade purchased from StanLab (Lublin, Poland). Acetonitrile (≥99.9%) for HPLC gradient grade and n-octanol (≥99%) were provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Disodium hydrogen phosphate dihydrate (≥99%) (Na₂HPO₄·2H₂O), disodium hydrogen phosphate dodecahydrate (99%) (Na₂HPO₄·12H₂O) were provided by Fisher Bioreagents (Pittsburgh, Pennsylvania, USA) and sodium dihydrogen phosphate anhydrous (98%) (NaH₂PO₄) was obtained from Acros Organics (Geel, Belgium). Potassium dihydrogen phosphate anhydrous (99%) was obtained from Merck (Darmstadt, Germany). Deuterated chloroform (CDCl₃) (99.8%) (+0.03% TMSCl) was purchased from Eurisotop (Cheshire, England).

Ossowicz P., Klebeko J., Janus E., Nowak A., Duchnik W., Kucharski Ł, & Klimowicz A. (2020). The effect of alcohols as vehicles on the percutaneous absorption and skin retention of ibuprofen modified with l-valine alkyl esters. RSC Advances, 10(68), 41727-41740.

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Optimized Purification of Ibuprofen

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All reagents were commercially available materials and were used without further purification. (RS)-Ibuprofen (99%) was obtained from Acros Organics (Geel, Belgium). L-valine and L-leucine (≥99%) were purchased from Carl Roth (Karlsruhe, Germany). Trimethylsilyl chloride (≥99%) (TMSCl) was provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Methanol, propan-2-ol (iPrOH), potassium chloride, sodium chloride, orthophosphoric acid (98%), pH = 4 buffer, citric acid, sodium hydroxide were high purity obtained from Chempur (Piekary Ślaskie, Poland). Ammonium hydroxide solution 25% (NH₃∙H₂O) was of analytical grade purchased from StanLab (Lublin, Poland). Acetonitrile (≥99.9%) for HPLC gradient grade was provided by Sigma-Aldrich (Steinheim am Albuch, Germany). Anhydrous disodium hydrogen phosphate (≥99%) (Na₂HPO₄), anhydrous potassium dihydrogen phosphate (99%) (KH₂PO₄) were provided by Merck (Darmstadt, Germany). Yeast extract and bacto-peptone were obtained from Graso BIOTECH (Starogard Gdański, Poland). D-mannitol (≥99.9%) was provided by POL-AURA (Dywity, Poland).

Ossowicz-Rupniewska P., Rakoczy R., Nowak A., Konopacki M., Klebeko J., Świątek E., Janus E., Duchnik W., Wenelska K., Kucharski Ł, & Klimowicz A. (2021). Transdermal Delivery Systems for Ibuprofen and Ibuprofen Modified with Amino Acids Alkyl Esters Based on Bacterial Cellulose. International Journal of Molecular Sciences, 22(12), 6252.

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HPLC Analysis of Phenolic Compounds

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Acetonitrile (Sigma-Aldrich, Poland), acetic acid (99.9% purity, Chempur, Poland), deionized water (Sigma-Aldrich, Warsaw, Poland), methanol (Merck, Warsaw, Poland), ortho-phosphoric acid (Chempur, Warsaw, Poland), and phenolic compound standards, including ferulic acid (CAS 537-98-4), chlorogenic acid (CAS 327-97-9), kaempferol-3-O-glucoside (CAS 480-10-4), quercetin-3-O-glucoside (CAS 482-35-9), quercetin-3-O-rutinoside (CAS 207671-50-9), myrycetin (CAS 529-44-2), cyanidin-3-O-glucoside (CAS: 7084-24-4), and cyjanidin-3-O-rutinoside (CAS 18719-76-1), were used in this study. (Sigma-Aldrich, Supelco, Poland).

Głowacka A., Rozpara E, & Hallmann E. (2020). The Dynamic of Polyphenols Concentrations in Organic and Conventional Sour Cherry Fruits: Results of a 4-Year Field Study. Molecules, 25(16), 3729.

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ABTS-based Antioxidant Capacity Assay

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ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (Sigma-Aldrich, Poland); acetonitrile (Sigma-Aldrich, Poland); deionized water (Sigma-Aldrich, Poland); ethyl acetate (Merck, Poland); methanol (Merck, Poland); ortho-phosphoric acid (Chempur, Poland); phenolic compound standards (purity 99.5%—99.9%), including caffeic acid, chlorogenic acid, epigallocatechin, gallic acid, kaempferol, kaempferol-3-O-glucoside, quercetin, quercetin-3-O-glucoside, quercetin-3-O-rutinoside, and salicylic acid (Sigma-Aldrich, Poland); caffeine (Merck, Poland); and phosphate-buffered saline (Merck, Poland) were used in this study.

Górecki M, & Hallmann E. (2020). The Antioxidant Content of Coffee and Its In Vitro Activity as an Effect of Its Production Method and Roasting and Brewing Time. Antioxidants, 9(4), 308.

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Comprehensive Analysis of Bioactive Compounds

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HPLC grade acetone, ethyl acetate, and meta-phosphoric acid were obtained from Sigma-Aldrich (Poznań, Poland). HPLC grade acetonitrile, HPLC grade methanol, ortho-phosphoric acid, and ultrapure magnesium carbonate were obtained from Chempur (Piekary Śląskie, Poland). Standards of β-carotene, caffeic acid, chlorogenic acid, chlorophyll a, chlorophyll b, dehydroascorbic acid, ferulic acid, gallic acid, kaempferol-3-O-glucoside, lutein, L-ascorbic acid, p-coumaric acid, and quercetin-3-O-rutinoside (HPLC grade, 99.5–99.9% pure) were obtained from Fluka and Sigma-Aldrich (Poznań, Poland). Standards of fructose (99.9% CAS 57-48-7), glucose (99.9% CAS 50-99-7), and sucrose (99.9% CAS 57-50-1) were obtained from Sigma-Aldrich (Warsaw, Poland).

Kopczyńska K., Średnicka-Tober D., Hallmann E., Wilczak J., Wasiak-Zys G., Wyszyński Z., Kucińska K., Perzanowska A., Szacki P., Barański M., Gawron P., Góralska-Walczak R., Rembiałkowska E, & Kazimierczak R. (2021). Bioactive Compounds, Sugars, and Sensory Attributes of Organic and Conventionally Produced Courgette (Cucurbita pepo). Foods, 10(10), 2475.

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Phenolic and Carotenoid Analysis Protocol

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The following chemicals were used in the procedures that follow: acetonitrile of HPLC purity (Sigma-Aldrich, Poland), deionized water (Sigma-Aldrich, Poland), ethyl acetate of HPLC purity, (Merck, Poland), methanol of HPLC purity (Merck, Poland), ortho-phosphoric acid 99.9% (Chempur, Poland), hydrochloric acid 36% (Chempur, Poland), phenolics standards (purity 99.5%-99.9%): gallic acid, chlorogenic acid, caffeic acid, p-coumaric acid, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, quercetin, pelargonidin-3,5-di-O-glucoside, peonidin-3,5-di-O-glucoside, petunin-3,5-di-O-glucoside (Sigma-Aldrich, Poland), lutein, zeaxanthin, β-carotene (Sigma-Aldrich, Poland).

Vaitkevičienė N., Kulaitienė J., Jarienė E., Levickienė D., Danillčenko H., Średnicka-Tober D., Rembiałkowska E., & Hallmann E. (2020). Characterization of Bioactive Compounds in Colored Potato (Solanum Tuberosum L.) Cultivars Grown with Conventional, Organic, and Biodynamic Methods. Sustainability, 12(7), 2701-2701.

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